Ababneh Nidaa A, Al-Kurdi Ban, Barham Raghda, Ali Dema, Sharar Nour, Abuarqoub Duaa, Jafar Hanan, Salah Bareqa, Awidi Abdalla
Cell Therapy Center, The University of Jordan, Amman, Jordan.
Cell Therapy Center, The University of Jordan, Amman, Jordan.
Stem Cell Res. 2020 Dec;49:102000. doi: 10.1016/j.scr.2020.102000. Epub 2020 Sep 28.
Mesenchymal stem cells (MSCs) are recognized as a valuable source of cells in clinical treatment and tissue engineering applications. In this study, we created human induced pluripotent stem cells (hiPSCs) from different MSC sources to evaluate the capacity of MSC-derived iPSCs to differentiate into any cell type of the human body and to serve as an alternative source for iPSC generation. Here in, the generated hiPSC lines retained their normal karyotype and showed similar STR-based identities to the parental cells. Reprogrammed cells also showed positive expression of the pluripotency markers and the ability to differentiate into the three germ layers.
间充质干细胞（MSCs）在临床治疗和组织工程应用中被认为是一种有价值的细胞来源。在本研究中，我们从不同的间充质干细胞来源创建了人类诱导多能干细胞（hiPSCs），以评估源自间充质干细胞的诱导多能干细胞分化为人体任何细胞类型的能力，并作为诱导多能干细胞生成的替代来源。在此，所产生的人类诱导多能干细胞系保留了其正常核型，并显示出与亲本细胞相似的基于短串联重复序列的身份。重编程细胞还显示多能性标志物的阳性表达以及分化为三个胚层的能力。
