Department of Animal Biology, Biology Institute, State Campinas University, Campinas, São Paulo, Brazil.
Department of Genetics and Evolution, Microbiology and Immunology, Biology Institute, State Campinas University, Campinas, São Paulo, Brazil.
Comp Immunol Microbiol Infect Dis. 2020 Dec;73:101551. doi: 10.1016/j.cimid.2020.101551. Epub 2020 Sep 17.
Human visceral leishmaniasis (VL) and canine leishmaniasis (CanL) in countries of South and Central America are caused by Leishmania infantum and has been endemic in Brazil for several years. The parasite biodiversity as well as the pharmacologic properties of drugs and the host species, are involved in the efficacy or inefficacy of leishmaniasis treatments. Although there are substantial number of reports describing the genetic characterization of the clinical field isolates of L. infantum,the phenotypic parameters have been less studied. In this study isolates from human and canine leishmaniasis (Hum1 and Can1) obtained in Campinas, São Paulo state, Brazil were identified as L. infantum. The Hum1 and Can1 isolates exhibited typical promastigote growth pattern. Regarding morphological features Can1 isolate differed in cell size. The infectivity in vitro of both isolatesis lower compared to the reference strain of L. infantum. Moreover, the in vivo infectivity of the three parasites is similar in Balb/c mice. The Hum1 isolate is more sensitive to leishmanial drugs (amphotericin B, miltefosine and glucantime) than the Can1 isolate when inside human macrophages, but not when inside canine macrophages. These findings indicated that L. infantum isolates differs in some phenotypic characteristics.
人体内脏利什曼病（VL）和犬内脏利什曼病（CanL）在南美洲和中美洲国家由利什曼原虫引起，并且在巴西已经流行了多年。寄生虫生物多样性以及药物和宿主物种的药理特性都参与了利什曼病治疗的效果或无效。尽管有大量描述利什曼原虫临床分离株遗传特征的报告，但对表型参数的研究较少。在这项研究中，从巴西坎皮纳斯获得的人体和犬内脏利什曼病（Hum1 和 Can1）分离株被鉴定为利什曼原虫。Hum1 和 Can1 分离株表现出典型的前鞭毛体生长模式。关于形态特征，Can1 分离株在细胞大小上有所不同。与利什曼原虫参考株相比，两种分离株的体外感染性较低。此外，在 Balb/c 小鼠体内，三种寄生虫的感染性相似。与 Can1 分离株相比，Hum1 分离株在人巨噬细胞内对利什曼原虫药物（两性霉素 B、米替福新和葡萄糖胺）更敏感，但在犬巨噬细胞内则不然。这些发现表明利什曼原虫分离株在一些表型特征上存在差异。
