Plasma Life Science and ZIK Plasmatis, Leibniz Institute for Plasma Science and Technology (INP), Felix-Hausdorff-Str. 2, 17489, Greifswald, Germany.
Plasma Life Science and ZIK Plasmatis, Leibniz Institute for Plasma Science and Technology (INP), Felix-Hausdorff-Str. 2, 17489, Greifswald, Germany; Department of General Visceral, Thoracic, and Vascular Surgery, Greifswald University Medical Center, Sauerbruchstr. DZ7, 17475, Greifswald, Germany.
Free Radic Biol Med. 2020 Dec;161:32-49. doi: 10.1016/j.freeradbiomed.2020.09.026. Epub 2020 Oct 1.
Cold plasma technology is an emerging tool facilitating the spatially controlled delivery of a multitude of reactive species (ROS) to the skin. While the therapeutic efficacy of plasma treatment has been observed in several types of diseases, the fundamental consequences of plasma-derived ROS on skin physiology remain unknown. We aimed to bridge this gap since the epidermal skin barrier and perfusion plays a vital role in health and disease by maintaining homeostasis and protecting from environmental damage. The intact skin of SKH1 mice was plasma-treated in vivo. Gene and protein expression was analyzed utilizing transcriptomics, qPCR, and Western blot. Immunofluorescence aided the analysis of percutaneous skin penetration of curcumin. Tissue oxygenation, perfusion, hemoglobin, and water index was investigated using hyperspectral imaging. Reversed-phase liquid-chromatography/mass spectrometry was performed for the identification of changes in the lipid composition and oxidation. Transcriptomic analysis of plasma-treated skin revealed modulation of genes involved in regulating the junctional network (tight, adherence, and gap junctions), which was confirmed using qPCR, Western blot, and immunofluorescence imaging. Plasma treatment increased the disaggregation of cells in the stratum corneum (SC) concomitant with increased tissue oxygenation, gap junctional intercellular communication, and penetration of the model drug curcumin into the SC preceded by altered oxidation of skin lipids and their composition in vivo. In summary, plasma-derived ROS modify the junctional network, which promoted tissue oxygenation, oxidation of SC-lipids, and restricted penetration of the model drug curcumin, implicating that plasma may provide a novel and sensitive tool of skin barrier regulation.
冷等离子体技术是一种新兴的工具，可实现多种活性物质（ROS）在空间上受控地递送至皮肤。虽然等离子体处理在几种疾病中已观察到治疗效果，但等离子体衍生的 ROS 对皮肤生理学的基本影响仍不清楚。我们旨在弥合这一差距，因为表皮皮肤屏障和灌注通过维持体内平衡和防止环境损伤，在健康和疾病中起着至关重要的作用。我们对 SKH1 小鼠的完整皮肤进行了体内等离子体处理。利用转录组学、qPCR 和 Western blot 分析了基因和蛋白质表达。免疫荧光辅助分析姜黄素的经皮皮肤渗透。利用高光谱成像研究组织氧合、灌注、血红蛋白和水指数。采用反相液相色谱/质谱法鉴定脂质组成和氧化的变化。对等离子体处理皮肤的转录组分析显示，参与调节连接网络（紧密、粘附和间隙连接）的基因发生了调制，这通过 qPCR、Western blot 和免疫荧光成像得到了证实。等离子体处理增加了角质层（SC）中细胞的解聚，同时伴随着组织氧合、间隙连接细胞间通讯的增加以及模型药物姜黄素渗透到 SC 中，这是由体内皮肤脂质的氧化和组成改变引起的。总之，等离子体衍生的 ROS 修饰了连接网络，促进了组织氧合、SC 脂质的氧化和模型药物姜黄素的渗透受限，这表明等离子体可能提供了一种新的、敏感的皮肤屏障调节工具。
