Department of Atomic and Molecular Physics, Faculty of Science, University of Mazandaran, Babolsar, Iran.
Plasma Technology Research Core, Faculty of Science, University of Mazandaran, Babolsar, Iran.
Sci Rep. 2023 Feb 4;13(1):2059. doi: 10.1038/s41598-023-28736-4.
Cold atmospheric pressure plasmas are considered a forthcoming method in many research areas. Plasma modification of biomolecules has received much attention in addition to plasma-treated biomaterials. Hence, in this work, we operated a transient spark plasma (TSP) discharge to study its effect on the L-cysteine chemical structure. the TSP was configured in a pin-to-ring electrode arrangement and flowed by Ar gas. We also investigated the effect of two chemicals; dimethyl sulfoxide (DMSO) and hydrogen peroxide (HO) by the bubbling method to show how they can change the creation of new chemical bioproducts. Ultraviolet-Visible absorption spectroscopy, Fourier transform infrared spectroscopy and Liquid chromatography-mass spectroscopy were used to investigate any changes in chemical bonds of cysteine structure and to depict the generation of new biomolecules. Based on the displayed results plasma-generated reactive species had a great role in the chemical structure of the cysteine. Entering DMSO and HO into the plasma caused the creation of new products and the heaviest biomolecule was produced by the simultaneous addition of DMSO and HO. The results also predicted that some chemical products and amino acids with a higher value molecular masse produced from the polymerization process of cysteine solution. The strong oxidation process is responsible for the heavy chemical compounds.
冷等离体子体被认为是许多研究领域的一种新兴方法。除了等离子体处理的生物材料外，生物分子的等离子体修饰也受到了广泛关注。因此，在这项工作中，我们操作了一个瞬态火花等离子体（TSP）放电来研究其对 L-半胱氨酸化学结构的影响。TSP 采用针-环电极配置，并通过 Ar 气体流动。我们还通过鼓泡法研究了两种化学物质；二甲基亚砜（DMSO）和过氧化氢（HO）的影响，以展示它们如何改变新的化学生物产物的生成。使用紫外-可见吸收光谱、傅里叶变换红外光谱和液相色谱-质谱法来研究半胱氨酸结构中化学键的任何变化，并描述新生物分子的生成。基于显示的结果，等离子体产生的活性物质对半胱氨酸的化学结构有很大的作用。将 DMSO 和 HO 引入等离子体中会导致新产物的生成，并且同时添加 DMSO 和 HO 会产生最重的生物分子。结果还预测，一些化学产物和具有更高分子量的氨基酸是由半胱氨酸溶液的聚合过程产生的。强氧化过程是产生重化学化合物的原因。
