鲤鱼水肿病毒病的分子特征：中国锦鲤（Cyprinus carpio）面临的新威胁。

Molecular characterization of carp edema virus disease: An emerging threat to koi Cyprinus carpio in China.

机构信息

Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin, 300384, China.

School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.

出版信息

Microb Pathog. 2020 Dec;149:104551. doi: 10.1016/j.micpath.2020.104551. Epub 2020 Oct 1.

DOI:10.1016/j.micpath.2020.104551

PMID:33011362

Abstract

Carp edema virus disease (CEVD) has resulted in great economic losses in koi (Cyprinus carpio koi) and common carp (Cyprinus carpio carpio) populations in the world. In this study, the diseased koi were diagnosed as CEV infection based on 5' untranslated region (5'UTR) and 4a protein genes by the conventional PCR, nested PCR and quantitative PCR (qPCR) analyses. Phylogenetic tree analysis showed that the TJ201708 strain was classified into the genogroup IIa. Furthermore, qPCR of 5'UTR gene revealed that the lowest detection limit was 4.0 fg/μL. The pathogenicity of CEV for koi was demonstrated in the infection experiments. Histopathological examination revealed the petechial hemorrhages of liver and spleen, vacuolization of lamina propria of intestine and swelling and necrosis of respiratory epithelial cells of gills. To our knowledge, this is the first report the qPCR of 5'UTR gene in the detection of carp edema virus.

摘要

锦鲤疱疹病毒病 (CEVD) 已导致世界范围内锦鲤 (Cyprinus carpio koi) 和鲤鱼 (Cyprinus carpio carpio) 种群遭受重大经济损失。在本研究中，通过常规 PCR、巢式 PCR 和定量 PCR（qPCR）分析，根据 5'非翻译区（5'UTR）和 4a 蛋白基因，将患病锦鲤诊断为 CEV 感染。系统进化树分析表明，TJ201708 株被分类为基因 IIa 组。此外，5'UTR 基因的 qPCR 显示最低检测限为 4.0 fg/μL。CEV 对锦鲤的致病性在感染实验中得到了证实。组织病理学检查显示肝脏和脾脏出现瘀点出血、肠固有层出现空泡化以及鳃呼吸上皮细胞肿胀和坏死。据我们所知，这是首次报道用 5'UTR 基因 qPCR 检测锦鲤疱疹病毒。