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即时多重聚合酶链反应比粪便培养提前 94 小时确定溶血尿毒综合征的病原菌。

Point-of-care multiplex-PCR enables germ identification in haemolytic uremic syndrome 94 h earlier than stool culture.

机构信息

Servicio de Terapia Intensiva Infantil, Hospital Pediátrico Dr. Humberto Notti, Mendoza, Argentina.

Servicio de Infectología, Hospital Pediátrico Dr. Humberto Notti, Mendoza, Argentina.

出版信息

Eur J Clin Microbiol Infect Dis. 2021 Mar;40(3):643-645. doi: 10.1007/s10096-020-04060-x. Epub 2020 Oct 4.

DOI:10.1007/s10096-020-04060-x
PMID:33011905
Abstract

Haemolytic uremic syndrome often affects children causing a relevant morbidity and mortality. We compared the time to diagnosis by multiplex-PCR and stool culture in 15 children from two centres. Multiplex-PCR accelerated the time to diagnosis by 94 (95% confidence interval, 80-119; P = 0.0007) hours. Multiplex-PCR offers a time advantage of stool culture that may aid in earlier identification of outbreak clusters.

摘要

溶血尿毒综合征常影响儿童，导致较高的发病率和死亡率。我们比较了两种方法（多重聚合酶链反应和粪便培养）在诊断 15 名儿童时的用时。多重聚合酶链反应将诊断用时缩短了 94 小时（95%置信区间：80-119；P=0.0007）。与粪便培养相比，多重聚合酶链反应在诊断时间上有优势，可能有助于更早识别暴发聚集。

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Contribution of the FilmArray Gastrointestinal Panel in the laboratory diagnosis of gastroenteritis in a cohort of children: a two-year prospective study.
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