The Key Laboratory of Dai and Southern Medicine of Xishuangbanna Dai Autonomous Prefecture, Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, No. 138 Xuanwei Avenue, Jinghong, 666100, Yunnan, China.
Biochem Genet. 2021 Feb;59(1):283-299. doi: 10.1007/s10528-020-10001-2. Epub 2020 Oct 3.
Aspidopterys obcordata var. obcordata, a medicinal plant endemic to China, is a narrowly distributed species and wild resources are extremely limited. To evaluate the genetic variability and degree of genetic divergence of A. obcordata var. obcordata, and to make rational scientific decisions on its harvest and germplasm conservation, we collected 122 samples from across nearly all of its distribution area and studied genetic diversity using inter-simple sequence repeats (ISSRs), sequence-related amplified polymorphisms (SRAPs), and a method combining the two techniques. The results revealed the high genetic diversity of A. obcordata var. obcordata, mainly due to its intra-population diversity, and the top two populations with the highest levels of intra-population diversity were ML and DH, individuals of which can serve as excellent germplasm candidates during the processing of germplasm screening and conservation. In general, the combining method was prior to the ISSR analyses and SRAP analyses results, except for a slight difference in the genetic structure of individual populations. Therefore, we suggest that a combination analysis of the two marker methods is ideal for evaluating the genetic diversity and genetic relationships of A. obcordata var. obcordata.
狭叶凤尾蕨（Aspidopterys obcordata var. obcordata），中国特有的药用植物，分布范围狭窄，野生资源极为有限。为评估狭叶凤尾蕨的遗传多样性和遗传分化程度，为其合理采收和种质资源保护提供科学决策依据，本研究采用 ISSR 和 SRAP 两种分子标记技术相结合的方法，对分布于狭叶凤尾蕨自然分布区的 122 个样本进行了遗传多样性分析。结果表明，狭叶凤尾蕨具有较高的遗传多样性，主要源于其居群内的多样性，居群内多样性最高的两个群体为 ML 和 DH，这两个群体的个体可作为优良的种质资源候选材料，用于后续的狭叶凤尾蕨种质资源筛选和保护。总体而言，结合两种标记方法的分析结果优于单独使用 ISSR 或 SRAP 分析的结果，除了个别居群的遗传结构存在微小差异。因此，建议采用两种标记方法相结合的分析方法，以评估狭叶凤尾蕨的遗传多样性和遗传关系。
