Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, China.
School of Agriculture and Biology, Shanghai Jiao Tong University, Key Laboratory of Urban Agriculture, Ministry of Agriculture, Shanghai, China.
Plant Cell Environ. 2021 Jan;44(1):130-142. doi: 10.1111/pce.13899. Epub 2020 Oct 19.
CONSTANS-LIKE (COL) family members are commonly implicated in light signal transduction during early photomorphogenesis. However, some of their functions remain unclear. Here, we propose a role for COL13 in hypocotyl elongation in Arabidopsis thaliana. We found that COL13 RNA accumulates at high levels in hypocotyls and that a disruption in the COL13 function via a T-DNA insertion or RNAi led to the formation of longer hypocotyls of Arabidopsis seedlings under red light. On the contrary, overexpression of COL13 resulted in the formation of shorter hypocotyls. Using various genetic, genomic, and biochemical assays, we proved that another COL protein, COL3, directly binds to the promoter of COL13, and the promoter region of COL3 was targeted by the transcription factor LONG HYPOCOTYL 5 (HY5), to form an HY5-COL3-COL13 regulatory chain for regulating hypocotyl elongation under red light. Additionally, further study demonstrated that COL13 interacts with COL3, and COL13 promotes the interaction between COL3 and CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1), suggesting a possible COP1-dependent COL3-COL13 feedback pathway. Our results provide new information regarding the gene network in mediating hypocotyl elongation.
CONSTANS-LIKE (COL) 家族成员通常在早期光形态建成过程中的光信号转导中被涉及。然而，它们的一些功能仍不清楚。在这里，我们提出 COL13 在拟南芥下胚轴伸长中的作用。我们发现 COL13 RNA 在下胚轴中高水平积累，并且通过 T-DNA 插入或 RNAi 破坏 COL13 功能导致拟南芥幼苗在红光下形成更长的下胚轴。相反，COL13 的过表达导致下胚轴变短。通过各种遗传、基因组和生化分析，我们证明另一种 COL 蛋白 COL3 直接与 COL13 的启动子结合，并且转录因子 LONG HYPOCOTYL 5 (HY5) 的启动子区域靶向 COL3 的启动子区域，形成 HY5-COL3-COL13 调控链，以在红光下调节下胚轴伸长。此外，进一步的研究表明 COL13 与 COL3 相互作用，并且 COL13 促进 COL3 与 CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) 之间的相互作用，表明可能存在 COP1 依赖的 COL3-COL13 反馈途径。我们的结果提供了关于调节下胚轴伸长的基因网络的新信息。
