Department of Microbiology, Chulalongkorn University, Faculty of Science.
Graduate School of Environmental Science, Hokkaido University.
J Gen Appl Microbiol. 2021 Feb 26;66(6):330-338. doi: 10.2323/jgam.2020.01.011. Epub 2020 Oct 2.
Aureobasidium pullulans YTP6-14 was demonstrated to be an excellent multiple biosurfactant producer utilizing cheap carbon sources available in Thailand, including glycerol and cassava flour hydrolysate. A. pullulans YTP6-14 maximally produced 1.81 g/l biosurfactant in an aqueous layer (BS-AQ) in a medium containing glycerol, and 7.37 or 6.37 g/l biosurfactant in a heavy oil layer (BS-HO) in cassava flour hydrolysate or a glucose containing medium, respectively. Each BS-AQ and BS-HO had critical micelle concentration values of 41.32 mg/l and 13.51 mg/l, and both biosurfactants formed a stable food oil emulsion and reduced the amount of biofilms formed by Streptococcus sobrinus and Streptococcus mutans. BS-AQ and BS-HO were mainly composed of liamocins or exophilins and massoia lactone, respectively.
蜡状芽胞杆菌 YTP6-14 被证明是一种能够利用泰国当地丰富的廉价碳源（包括甘油和木薯淀粉水解液）高效生产多种生物表面活性剂的菌株。在含有甘油的培养基中，蜡状芽胞杆菌 YTP6-14 可在水相（BS-AQ）中最大产量 1.81 g/L 的生物表面活性剂，而在木薯淀粉水解液或含葡萄糖的培养基的重油相中（BS-HO），则可分别产量 7.37 或 6.37 g/L 的生物表面活性剂。BS-AQ 和 BS-HO 的临界胶束浓度值分别为 41.32 mg/L 和 13.51 mg/L，两种生物表面活性剂均可形成稳定的食用油脂乳液，并减少变形链球菌和变异链球菌形成的生物膜量。BS-AQ 和 BS-HO 主要由利亚莫宁或外生孢菌素和马索亚内酯组成。
