Faculty of Engineering, Department of Food Engineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey.
Faculty of Engineering, Department of Chemical Engineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey.
Arch Microbiol. 2024 Jul 16;206(8):353. doi: 10.1007/s00203-024-04065-6.
Liamocins are molecules with a polyol lipid structure produced by rare strains of Aureobasidium pullulans. In recent years, liamocins have attracted attention due to their antibacterial, anticancer and surface-active properties, and promising potential applications have been identified in the food, agriculture, medical and pharmaceutical industries. This study is the first to investigate the effects of different carbon and nitrogen sources on the growth and liamocin production kinetics of A. pullulans NBRC 100716 strain. This strain was selected among six different A. pullulans strains whose liamocin productions were tested by us for the first time. In fermentations carried out in shaking water baths, the carbon source that most supported the liamocin production of this strain was fructose, and the nitrogen source was peptone-yeast extract combination. In the medium containing fructose and the peptone-yeast extract mixture, A. pullulans NBRC 100716 produced 4.26 g liamocin L. The specific liamocin production rate (qp) of the strain in this medium was 0.0090 g liamocin/g mo.h. This study is also the first to produce liamocin with a fructophilic A. pullulans strain. Present findings in this research also demonstrated the excellent biosurfactant capacity of the liamocin produced by this strain. The obtained liamocin reduced the water surface tension to a degree that can compete with synthetic surfactants. Furthermore, this is the first report to reveal that the fatty acid profile of liamocin obtained from A. pullulans NBRC 100716 contains an appreciable amount of unsaturated fatty acids and is similar to the composition of vegetable oil.
利亚莫辛是一种具有多醇脂质结构的分子，由罕见的出芽短梗霉菌株产生。近年来，利亚莫辛因其具有抗菌、抗癌和表面活性特性而受到关注，并在食品、农业、医疗和制药行业中确定了有前途的潜在应用。本研究首次调查了不同碳源和氮源对出芽短梗霉 NBRC 100716 菌株生长和利亚莫辛生产动力学的影响。该菌株是从我们首次测试利亚莫辛生产的六种不同出芽短梗霉菌株中选择的。在摇瓶水浴发酵中，最支持该菌株利亚莫辛生产的碳源是果糖，氮源是蛋白胨-酵母提取物混合物。在含有果糖和蛋白胨-酵母提取物混合物的培养基中，出芽短梗霉 NBRC 100716 产生了 4.26 g 利亚莫辛 L。该菌株在该培养基中的比利亚莫辛产生率（qp）为 0.0090 g 利亚莫辛/g mo.h。本研究也是首次用果糖嗜生性出芽短梗霉菌株生产利亚莫辛。本研究的现有发现还证明了该菌株产生的利亚莫辛具有出色的生物表面活性剂能力。获得的利亚莫辛将水的表面张力降低到可与合成表面活性剂竞争的程度。此外，这是第一个报道揭示出芽短梗霉 NBRC 100716 获得的利亚莫辛的脂肪酸谱含有相当数量的不饱和脂肪酸，并且与植物油的组成相似。
