Biochemistry Unit, Department of Medical Sciences, Faculty of Science, Rangsit University, 52/347 Muang Ake, Phaholyothin Rd., Lakhok, Pathumthani, 12000, Thailand.
Biotechnol Lett. 2011 Jun;33(6):1151-7. doi: 10.1007/s10529-011-0548-1. Epub 2011 Feb 4.
From a survey of more than 50 diverse strains of Aureobasidium pullulans, 21 produced extracellular heavy oils. Most oil producers fell into phylogenetic clades 8, 9, and 11. Oil colors ranged from bright yellow to malachite. More than half of the strains produced oil that was fluorescent. In medium containing 5% (w/v) sucrose, oil yields ranged from 0.5 to 6 g oil/l. Strain CU 43 reached stationary growth phase at day 4 while oil yields were maximal at day 6. CU 43 produced bright yellow, highly fluorescent oil that also was visible as intracellular droplets under fluorescent microscopy. Oil was surface active, suggesting that it functions as a biosurfactant. Oil from two strains (CU 43 and NRRL Y-12974) differentially inhibited mammalian cancer cell lines. MALDI-TOF MS spectra suggested that A. pullulans strains produce a family of related oil structures.
从对 50 多种不同的出芽短梗霉菌株的调查中发现，有 21 种产生了细胞外重油。大多数产油菌株属于系统发育枝 8、9 和 11。油的颜色从亮黄色到孔雀石绿不等。超过一半的菌株产生的油具有荧光性。在含有 5%（w/v）蔗糖的培养基中，油的产量范围为 0.5 至 6 g 油/L。菌株 CU 43 在第 4 天达到静止生长阶段，而在第 6 天油产量达到最大值。CU 43 产生亮黄色、高荧光的油，在荧光显微镜下也可以看到它作为细胞内液滴的存在。油具有表面活性，表明它起生物表面活性剂的作用。来自两个菌株（CU 43 和 NRRL Y-12974）的油差异抑制哺乳动物癌细胞系。MALDI-TOF MS 谱表明，出芽短梗霉菌株产生了一系列相关的油结构。
