Lee Gun-Hwi, Hwang Kyung-A, Kang Ji-Houn, Choi Kyung-Chul
Laboratory of Biochemistry and Immunology (Lee, Hwang, Choi) and Laboratory of Veterinary Internal Medicine and Neuroendocrinology (Kang), College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, 28644, Republic of Korea.
Can J Vet Res. 2020 Oct;84(4):294-301.
Nakai is a medicinal herb found widely distributed throughout Korea. The biological activities of are well-documented and include anti-fungal, anti-inflammatory, and immunity enhancement. The objective of the present study was to investigate the immune-related activities of extract in dogs. The extract was acquired by ethanol extraction and purified by filtration. To examine the effect of extract on immune cell viability, human lymphocytes, such as Jurkat T-cells and Ramos B-cells, were exposed to the extract. After treatment with the extract, the number of Ramos B-cells was increased, whereas Jurkat T-cells remained unaffected. Griess assay revealed decreased nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated mouse macrophage Raw 264.7 cells after exposure to extract. To evaluate the effect in dogs, feed containing extract was provided to 8 dogs for 2 months. Blood samples were collected before, during, and after consumption of the feed. Peripheral blood mononuclear cells (PBMCs) were isolated from the blood samples and the number of T-cells and B-cells were assessed using flow cytometry with anti-dog fluorescein isothiocyanate (FITC)-conjugated CD3 and anti-dog phycoerythrin (PE)-conjugated CD21 antibodies, respectively. We observed a significant increase in the average number of B-cells in the PBMCs during ingestion of the feed containing . In addition, enzyme-linked immunosorbent assay (ELISA) revealed a decrease in the levels of tumor necrosis factor-alpha (TNF-α), a pro-inflammatory cytokine, in 3 out of 8 dogs and increased levels of interleukin-10 (IL-10), an anti-inflammatory cytokine, in 4 out of 8 dogs. Taken together, we believe that these changes indicate that extract is beneficial in improving the immunity of dogs by stimulating B-cells and inducing production of anti-inflammatory responses.
中井是一种在韩国广泛分布的草药。其生物活性有充分的文献记载，包括抗真菌、抗炎和增强免疫力。本研究的目的是调查中井提取物对犬类免疫相关的活性。提取物通过乙醇提取获得，并通过过滤进行纯化。为了检测中井提取物对免疫细胞活力的影响，将人淋巴细胞，如Jurkat T细胞和Ramos B细胞，暴露于该提取物中。用提取物处理后，Ramos B细胞数量增加，而Jurkat T细胞未受影响。格里斯试验显示，暴露于中井提取物后，脂多糖（LPS）刺激的小鼠巨噬细胞Raw 264.7细胞中一氧化氮（NO）的产生减少。为了评估中井提取物对犬类的作用，给8只犬提供含中井提取物的饲料，持续2个月。在喂食饲料前、期间和之后采集血样。从血样中分离出外周血单核细胞（PBMC），并分别使用抗犬异硫氰酸荧光素（FITC）偶联的CD3和抗犬藻红蛋白（PE）偶联的CD21抗体，通过流式细胞术评估T细胞和B细胞的数量。我们观察到，在摄入含中井提取物的饲料期间，PBMC中B细胞的平均数量显著增加。此外，酶联免疫吸附测定（ELISA）显示，8只犬中有3只促炎细胞因子肿瘤坏死因子-α（TNF-α）水平下降，8只犬中有4只抗炎细胞因子白细胞介素-10（IL-10）水平升高。综上所述，我们认为这些变化表明中井提取物通过刺激B细胞和诱导抗炎反应的产生，对提高犬类免疫力有益。
