Song Chunyuan, Zhang Jingjing, Liu Yang, Guo Xiangyin, Guo Yan, Jiang Xinyu, Wang Lianhui
Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergistic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, Nanjing, 210023, China.
Sens Actuators B Chem. 2020 Dec 15;325:128970. doi: 10.1016/j.snb.2020.128970. Epub 2020 Sep 28.
Pathogenic viruses with worldwide distribution, high incidence and great harm are significantly and increasingly threatening human health. However, there is still lack of sufficient, highly sensitive and specific detection methods for on-time and early diagnosis of virus infection. In this work, taking dengue virus (DENV) as an example, a highly sensitive SERS assay of DENV gene was proposed via a cascade signal amplification strategy of localized catalytic hairpin assembly (LCHA) and hybridization chain reaction (HCR). The SERS assay was performed by two steps, i.e., the operation of cascade signal amplification strategy and the following SERS measurements by transferring the products on SERS-active AgNRs arrays. The sensitivity of the cascade signal amplification strategy is significantly amplified, which is 4.5 times that of individual CHA, and the signal-to-noise ratio is also improved to 5.4 relative to 1.8 of the CHA. The SERS sensing possesses a linear calibration curve from 1 fM to 10 nM with the limit of detection low to 0.49 fM, and has good specificity, uniformity and recovery, which indicates that the highly sensitive SERS assay provides an attractive tool for reliable, early diagnosis of DENV gene and is worth to be popularized in a wide detection of other viruses.
分布于全球、发病率高且危害大的致病病毒正日益严重地威胁着人类健康。然而，对于病毒感染的及时和早期诊断，仍然缺乏足够的、高灵敏度和特异性的检测方法。在这项工作中，以登革病毒（DENV）为例，通过局部催化发夹组装（LCHA）和杂交链式反应（HCR）的级联信号放大策略，提出了一种高灵敏度的DENV基因表面增强拉曼光谱（SERS）检测方法。该SERS检测方法分两步进行，即级联信号放大策略的操作以及随后通过将产物转移到具有SERS活性的银纳米棒（AgNRs）阵列上进行SERS测量。级联信号放大策略的灵敏度显著提高，是单个催化发夹组装（CHA）的4.5倍，信噪比也从CHA的1.8提高到了5.4。该SERS传感在1 fM至10 nM范围内具有线性校准曲线，检测限低至0.49 fM，并且具有良好的特异性、均匀性和回收率，这表明该高灵敏度SERS检测方法为可靠、早期诊断DENV基因提供了一种有吸引力的工具，值得在其他病毒的广泛检测中推广。
