State Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications, Nanjing, 210023, China.
Guangzhou KingMed Center for Clinical Laboratory Co., Ltd, 10 Luoxuan 3rd Road, Guangzhou International Biotech Island, Guangdong, 510005, Guangdong, China; Guangzhou Laboratory, No.9 XingDaoHuanBei Road, Guangzhou International Bio Island, Guangzhou, 510005, Guangdong Province, China.
Biosens Bioelectron. 2022 Sep 15;212:114379. doi: 10.1016/j.bios.2022.114379. Epub 2022 May 16.
The development of rapid and ultra-sensitive detection technology of SARS-CoV-2 RNA for shortening the diagnostic window and achieving early detection of virus infections is a huge challenge to the efficient prevention and control of COVID-19. Herein, a novel ultra-sensitive surface-enhanced Raman spectroscopy (SERS) sensor powered by non-enzymatic signal amplification is proposed for rapid and reliable assay of SARS-CoV-2 RNA based on SERS-active silver nanorods (AgNRs) sensing chips and a specially designed smart unlocking-mediated target recycling signal amplification strategy. The SERS sensing was carried out by a one-pot hybridization of the lock probes (LPs), hairpin DNAs and SERS tags with SARS-CoV-2 RNA samples on an arrayed SERS sensing chip to achieve the recognition of SARS-CoV-2 RNA, the execution of nuclease-free unlocking-mediated target recycling signal amplification, and the combination of SERS tags to generate SERS signal. The SERS sensor for SARS-CoV-2 RNA can be achieved within 50 min with an ultra-high sensitivity low to 51.38 copies/mL, and has good selectivity in discriminating SARS-CoV-2 RNA against other respiratory viruses in representative clinical samples, which is well adapted for rapid, ultra-sensitive, multi-channel and point-of-care testing of viral nucleic acids, and is expected to achieve detection of SARS-CoV-2 infection in earlier detection windows for efficient COVID-19 prevention and control.
开发 SARS-CoV-2 RNA 的快速和超灵敏检测技术，以缩短诊断窗口期并实现病毒感染的早期检测，这对 COVID-19 的有效防控是一个巨大的挑战。在此，提出了一种基于 SERS 活性银纳米棒（AgNRs）传感芯片和专门设计的智能解锁介导的靶标循环信号放大策略的新型超灵敏表面增强拉曼光谱（SERS）传感器，用于 SARS-CoV-2 RNA 的快速可靠分析。SERS 传感是通过一锅法将锁探针（LPs）、发夹 DNA 和 SERS 标记物与 SARS-CoV-2 RNA 样品在阵列式 SERS 传感芯片上杂交来进行的，以实现对 SARS-CoV-2 RNA 的识别、无核酸酶解锁介导的靶标循环信号放大的执行以及 SERS 标记物的组合以产生 SERS 信号。SARS-CoV-2 RNA 的 SERS 传感器可以在 50 分钟内实现，检测限低至 51.38 拷贝/mL，具有很好的选择性，可以区分代表性临床样本中的 SARS-CoV-2 RNA 与其他呼吸道病毒，非常适合用于病毒核酸的快速、超灵敏、多通道和即时检测，有望在更早的检测窗口内实现 SARS-CoV-2 感染的检测，从而有效防控 COVID-19。
