Yu Lean Huat, Teh Cindy Shuan Ju, Yap Kien Pong, Thong Kwai Lin
Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Aquac Int. 2020;28(6):2547-2559. doi: 10.1007/s10499-020-00610-4. Epub 2020 Sep 26.
A unique strain of (designated as VP) causes acute hepatopancreatic necrosis disease (AHPND), a deadly bacterial disease associated with mass mortality in cultured shrimps since 2009. AHPND is responsible for severe economic losses worldwide, causing multimillion-dollar loss annually. Because of the rapid and high mortality rates in shrimps, substantial research has been carried out to develop rapid detection techniques. Also, recent technological advances such as the next-generation sequencing (NGS) have made it possible to elucidate relevant information about a pathogen in a single assay. This review summarizes the current research pertaining to VP, focusing on diagnosis and contribution of NGS technologies in the genomic studies of AHPND.
一种独特的菌株（命名为VP）会引发急性肝胰腺坏死病（AHPND），这是一种自2009年以来与养殖虾大规模死亡相关的致命细菌性疾病。AHPND在全球范围内造成了严重的经济损失，每年导致数百万美元的损失。由于虾类的死亡率迅速且很高，人们已经开展了大量研究来开发快速检测技术。此外，诸如新一代测序（NGS）等最新技术进展使得在一次检测中阐明有关病原体的相关信息成为可能。本综述总结了目前与VP相关的研究，重点关注NGS技术在AHPND基因组研究中的诊断和贡献。
