Low Dimensional Materials Research Centre (LDMRC), Department of Physics, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Sci Rep. 2018 Jan 17;8(1):896. doi: 10.1038/s41598-017-18825-6.
The exciting discovery of the semiconducting-like properties of deoxyribonucleic acid (DNA) and its potential applications in molecular genetics and diagnostics in recent times has resulted in a paradigm shift in biophysics research. Recent studies in our laboratory provide a platform towards detecting charge transfer mechanism and understanding the electronic properties of DNA based on the sequence-specific electronic response, which can be applied as an alternative to identify or detect DNA. In this study, we demonstrate a novel method for identification of DNA from different shrimp viruses and bacteria using electronic properties of DNA obtained from both negative and positive bias regions in current-voltage (I-V) profiles. Characteristic electronic properties were calculated and used for quantification and further understanding in the identification process. Aquaculture in shrimp industry is a fast-growing food sector throughout the world. However, shrimp culture in many Asian countries faced a huge economic loss due to disease outbreaks. Scientists have been using specific established methods for detecting shrimp infection, but those methods do have their significant drawbacks due to many inherent factors. As such, we believe that this simple, rapid, sensitive and cost-effective tool can be used for detection and identification of DNA from different shrimp viruses and bacteria.
脱氧核糖核酸（DNA）的半导体性质及其在分子遗传学和诊断学中的潜在应用的令人兴奋的发现，导致了生物物理学研究的范式转变。最近我们实验室的研究为基于序列特异性电子响应检测电荷转移机制和理解 DNA 的电子性质提供了一个平台，可作为替代方法来识别或检测 DNA。在这项研究中，我们展示了一种使用电流-电压（I-V）曲线中正负偏置区域获得的 DNA 的电子性质来识别不同虾病毒和细菌的 DNA 的新方法。计算了特征电子性质，并用于定量和进一步理解识别过程。虾养殖业是世界范围内快速发展的食品行业。然而，由于疾病爆发，许多亚洲国家的虾养殖业遭受了巨大的经济损失。科学家们一直在使用特定的已建立方法来检测虾的感染，但由于许多内在因素，这些方法确实存在显著的缺点。因此，我们相信这种简单、快速、灵敏和具有成本效益的工具可用于检测和识别不同虾病毒和细菌的 DNA。
