Hussien Yasmeen A, Abdalkadim Hussien, Mahbuba Waddah, Hadi Najah R, Jamil Dina A, Al-Aubaidy Hayder A
College of Pharmacy, Al-Kafeel University, Al-Najaf, Iraq.
Faculty of Medicine, University of Kufa, Al-Najaf, Iraq.
Indian J Clin Biochem. 2020 Oct;35(4):474-481. doi: 10.1007/s12291-019-00848-7. Epub 2019 Sep 13.
Acute kidney injury (AKI) is characterized by fast decline in renal function within a short period of time. Renal ischemic-reperfusion (I-R) injury is the main cause of AKI. This study aims to investigate the possible nephroprotective effect of lycopene on renal ischemic-reperfusion injury in mice model. Forty Swiss Albino adult male mice were randomly allocated onto one of the four study groups: sham group: mice had median laparotomy under anesthesia with no procedures performed, renal tissues and blood samples were collected. ischemic-reperfusion group (I-R-control): mice underwent median laparotomy under anesthesia, followed by 30 min bilateral renal ischemia. Renal tissues and blood samples were collected after 2 h from reperfusion. Vehicle-treated group: mice were pretreated with intra 1% dimethyl sulfoxide 30 min before inducing ischemia. Lycopene-treated group: mice were pretreated with 10 mg/kg intraperitoneal injection of lycopene 30 min before inducing renal ischemia. Renal tissues, and blood samples were collected after 2 h from reperfusion. Blood and tissue samples were collected to look for evidence of inflammation and necrosis. Blood urea nitrogen, serum creatinine as well as plasma NGAL levels were significantly increased in the active control group ( ≤ 0.05), when compared to the sham group. Similarly, renal levels of Notch2/Hes 1, TLR 2, IL-6, Bax, and F2-isoprostane were significantly increased in the active control group as compared to the sham group ( ≤ 0.05). Moreover, lycopene treatment was found to be significantly effective in reducing the increased levels of these markers after I-R injury ( ≤ 0.05).
急性肾损伤（AKI）的特征是肾功能在短时间内迅速下降。肾缺血再灌注（I-R）损伤是AKI的主要原因。本研究旨在探讨番茄红素对小鼠模型肾缺血再灌注损伤可能的肾保护作用。40只成年瑞士白化雄性小鼠被随机分配到四个研究组之一：假手术组：小鼠在麻醉下进行正中剖腹术，不进行任何操作，收集肾组织和血液样本。缺血再灌注组（I-R对照组）：小鼠在麻醉下进行正中剖腹术，随后进行30分钟的双侧肾缺血。再灌注2小时后收集肾组织和血液样本。溶剂处理组：在诱导缺血前30分钟，小鼠腹腔注射1%二甲基亚砜进行预处理。番茄红素处理组：在诱导肾缺血前30分钟，小鼠腹腔注射10mg/kg番茄红素进行预处理。再灌注2小时后收集肾组织和血液样本。收集血液和组织样本以寻找炎症和坏死的证据。与假手术组相比，活性对照组的血尿素氮、血清肌酐以及血浆中性粒细胞明胶酶相关脂质运载蛋白（NGAL）水平显著升高（≤0.05）。同样，与假手术组相比，活性对照组的肾组织中Notch2/Hes 1、Toll样受体2（TLR 2）、白细胞介素-6（IL-6）、Bax和F2-异前列腺素水平显著升高（≤0.05）。此外，发现番茄红素治疗对降低I-R损伤后这些标志物升高的水平具有显著效果（≤0.05）。
