Department of Biochemistry, Faculty of Science, University of Allahabad, Allahabad, Uttar Pradesh, India.
Department of Biochemistry, Female Center for Scientific and Medical Colleges, College of Science, King Saud University, Riyadh, Saudi Arabia.
J Cell Physiol. 2019 Aug;234(10):18329-18343. doi: 10.1002/jcp.28466. Epub 2019 Mar 19.
Exposure to mixture of pesticides in agricultural practices pose a serious threat to the nontarget animals. In present work, we have evaluated the synergistic effect of cartap and malathion on rat liver followed by impact of Aloe vera leaves aqueous extract, which is not known. The animals in eight groups were used; each containing six rats: Group 1 acted as a control, Group 2-control with A. vera leaves aqueous extract, Group 3-with cartap, Group 4-with malathion, Group 5-with mixture of cartap and malathion, Group 6-cartap with the pretreatment of A. vera leaf extract, Group 7-malathion with the pretreatment of A. vera leaf extract, Group 8-mixture of cartap and malathion with the pretreatment of A. vera leaf extract . The animals treated for 15 days were killed after 24hr of last treatment. The biochemical studies in the rat liver demonstrated significant perturbations in the levels of nonenzymatic (glutathione and malondialdehyde) and enzymatic (superoxide dismutase, catalase, and glutathione- S-transferase) antioxidative indices. The histopathological examination of liver revealed serious congestion in central vein and the disorganization of hepatic cords due to pesticide treatment. The administration of A. vera leaves aqueous extract was able to markedly protect rat liver from the pesticides-induced toxicity. The data indicated that pesticides were able to significantly induce oxidative stress which was substantially reduced by the application of plant extract .
农业实践中接触混合农药对非目标动物构成严重威胁。在本工作中，我们评估了 cartap 和马拉硫磷对大鼠肝脏的协同作用，随后评估了其对库拉索芦荟叶水提物的影响，这一点尚不清楚。使用了 8 组动物，每组包含 6 只大鼠：第 1 组作为对照，第 2 组为对照加库拉索芦荟叶水提物，第 3 组用 cartap，第 4 组用马拉硫磷，第 5 组用 cartap 和马拉硫磷混合物，第 6 组用 cartap 预处理库拉索芦荟叶提取物，第 7 组用马拉硫磷预处理库拉索芦荟叶提取物，第 8 组用 cartap 和马拉硫磷混合物预处理库拉索芦荟叶提取物。第 15 天治疗结束后 24 小时处死动物。大鼠肝生化研究表明非酶（谷胱甘肽和丙二醛）和酶（超氧化物歧化酶、过氧化氢酶和谷胱甘肽-S-转移酶）抗氧化指标水平显著失调。肝组织病理学检查显示，农药处理导致中央静脉严重充血和肝索排列紊乱。库拉索芦荟叶水提物的给药能够显著保护大鼠肝脏免受农药诱导的毒性。数据表明，农药能够显著诱导氧化应激，而植物提取物的应用可显著降低氧化应激。
