Pereira Bruna L B, Reis Patrícia P, Severino Fábio E, Felix Tainara F, Braz Mariana G, Nogueira Flávia R, Silva Renata A C, Cardoso Ana C, Lourenço Maria A M, Figueiredo Amanda M, Chiuso-Minicucci Fernanda, Azevedo Paula S, Polegato Bertha F, Okoshi Katashi, Fernandes Ana A H, Paiva Sergio A R, Zornoff Leonardo A M, Minicucci Marcos F
Internal Medicine Department, Botucatu Medical School, Univ Estadual Paulista (UNESP), Botucatu, Brazil.
Department of Surgery and Orthopedics, Botucatu Medical School, Univ Estadual Paulista (UNESP), Botucatu, Brazil.
J Nutr Biochem. 2017 Aug;46:117-124. doi: 10.1016/j.jnutbio.2017.05.010. Epub 2017 Jun 1.
The objective of this study was to evaluate the influence of tomato or lycopene supplementation on cardiac remodeling after myocardial infarction (MI). Male Wistar rats were assigned to four groups: the sham group (animals that underwent simulated surgery) that received a standard chow (S; n=18), the infarcted group that received a standard chow (MI; n=13), the infarcted group supplemented with lycopene (1 mg of lycopene/kg body weight/day) (MIL; n=16) and the infarcted group supplemented with tomato (MIT; n=16). After 3 months, morphological, functional and biochemical analyses were performed. The groups MIL and MIT showed decreased interstitial fibrosis induced by infarction. Tomato supplementation attenuated the hypertrophy induced by MI. In addition, tomato and lycopene improved diastolic dysfunction evaluated by echocardiographic and isolated heart studies, respectively. The MI group showed higher levels of cardiac TNF-α compared to the MIL and MIT groups. Decreased nuclear factor E2-related factor 2 was measured in the MIL group. Lipid hydroperoxide levels were higher in the infarcted groups; however, the MIT group had a lower concentration than did the MI group [S=223±20.8, MI=298±19.5, MIL=277±26.6, MIT=261±28.8 (nmol/g); n=8; P<.001]. We also examined left ventricle miRNA expression; when compared to the S group, the MIL group uniquely down-regulated the expression of eight miRNAs. No miRNA was found to be up-regulated uniquely in the MIT and MIL groups. In conclusion, tomato or lycopene supplementation attenuated the cardiac remodeling process and improved diastolic function after MI. However, the effect of lycopene and tomato supplementation occurred through different mechanistic pathways.
本研究的目的是评估补充番茄或番茄红素对心肌梗死后心脏重构的影响。将雄性Wistar大鼠分为四组：接受标准饲料的假手术组（进行模拟手术的动物）（S组；n = 18）、接受标准饲料的梗死组（MI组；n = 13）、补充番茄红素（1毫克番茄红素/千克体重/天）的梗死组（MIL组；n = 16）和补充番茄的梗死组（MIT组；n = 16）。3个月后，进行形态学、功能和生化分析。MIL组和MIT组梗死诱导的间质纤维化减少。补充番茄减轻了MI诱导的心肌肥厚。此外，番茄和番茄红素分别通过超声心动图和离体心脏研究改善了舒张功能障碍。与MIL组和MIT组相比，MI组心脏TNF-α水平更高。MIL组中核因子E2相关因子2的水平降低。梗死组的脂质过氧化水平较高；然而，MIT组的浓度低于MI组[S = 223±20.8，MI = 298±19.5，MIL = 277±26.6，MIT = 261±28.8（nmol/g）；n = 8；P <.001]。我们还检测了左心室miRNA表达；与S组相比，MIL组独特地下调了8种miRNA的表达。在MIT组和MIL组中未发现独特上调的miRNA。总之，补充番茄或番茄红素可减轻MI后的心脏重构过程并改善舒张功能。然而，补充番茄红素和番茄的作用是通过不同的机制途径实现的。
