血清白细胞介素-2 水平升高与活动期类风湿关节炎患者外周血自然杀伤细胞水平异常相关。
Increased Serum Interleukin-2 Levels Are Associated with Abnormal Peripheral Blood Natural Killer Cell Levels in Patients with Active Rheumatoid Arthritis.
机构信息
Department of Rheumatology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China.
Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
出版信息
Mediators Inflamm. 2020 Sep 15;2020:6108342. doi: 10.1155/2020/6108342. eCollection 2020.
OBJECTIVE
To investigate the relationship between serum interleukin-2 (IL-2) levels and disease activity, absolute numbers of peripheral lymphocyte subsets, autoantibodies, and associated cytokines in patients with rheumatoid arthritis (RA).
METHODS
This study included 106 patients with RA, evaluated their disease activity (DAS28 score), and divided them into disease remission (DAS28 ≤ 2.6), low disease activity (DAS28 ≤ 3.2), and moderate-high disease activity (28 > 3.2) groups. Flow cytometry was used to detect the absolute numbers of peripheral lymphocyte subpopulations and CD4+ T cell subsets in each group, and serum cytokine levels were measured using cytometric bead array.
RESULTS
Serum IL-2 levels in RA patients were positively correlated with disease activity and rheumatoid factor titers ( < 0.001 and = 0.045, respectively), and multiple regression analysis revealed that serum IL-2 levels were an independent factor affecting disease activity. Serum IL-2 levels were positively correlated with Th17/Treg ratios ( = 0.013). Compared with the remission group, peripheral lymphocyte and CD4+ T lymphocyte subsets in patients with active RA decreased to varying degrees; however, the numbers of peripheral natural killer (NK) cells were significantly higher in the moderate-high disease activity group than in the remission ( = 0.046) and low disease activity ( = 0.020) groups; the percentages of NK cells had the same trend. In addition, the number and percentage of NK cells were positively correlated with serum IL-2 levels ( = 0.018 and = 0.006, respectively).
CONCLUSIONS
In RA patients, serum IL-2 levels were not only correlated with patients' disease activity and autoantibody levels but were also involved in their Th17/Treg immune imbalance. In addition, in patients with active RA, NK cell levels were abnormally elevated, possibly due to high serum levels of IL-2.
目的
探讨类风湿关节炎（RA）患者血清白细胞介素-2（IL-2）水平与疾病活动度、外周血淋巴细胞亚群绝对计数、自身抗体及相关细胞因子的关系。
方法
纳入 106 例 RA 患者，评估其疾病活动度（DAS28 评分），并分为疾病缓解组（DAS28≤2.6）、低疾病活动组（DAS28≤3.2）和中高度疾病活动组（28＞3.2）。采用流式细胞术检测各组外周血淋巴细胞亚群及 CD4+T 细胞亚群绝对计数，采用流式细胞术检测血清细胞因子水平。
结果
RA 患者血清 IL-2 水平与疾病活动度及类风湿因子滴度呈正相关（均＜0.001 和 =0.045），多元逐步回归分析显示血清 IL-2 水平是影响疾病活动度的独立因素。血清 IL-2 水平与 Th17/Treg 比值呈正相关（ =0.013）。与缓解组比较，活动期 RA 患者外周血淋巴细胞及 CD4+T 淋巴细胞亚群均不同程度下降，而中高度疾病活动组外周自然杀伤（NK）细胞数显著高于缓解组（ =0.046）和低疾病活动组（ =0.020），NK 细胞百分比也有相同趋势。此外，NK 细胞数及百分比与血清 IL-2 水平呈正相关（ =0.018 和 =0.006）。
结论
在 RA 患者中，血清 IL-2 水平不仅与患者的疾病活动度和自身抗体水平相关，还与 Th17/Treg 免疫失衡有关。此外，活动期 RA 患者 NK 细胞水平异常升高，可能与血清 IL-2 水平升高有关。
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