• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

SIRT1是2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷减轻人脐静脉内皮细胞衰老的靶基因。

SIRT1 Is the Target Gene for 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-Glucoside Alleviating the HUVEC Senescence.

作者信息

Guo Yan, Fan Wenxue, Xie Yuefeng, Cao Shuyu, Wan Haitong, Jin Bo

机构信息

College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.

College of Basic Medicine and Public Health, Zhejiang Chinese Medical University, Hangzhou, China.

出版信息

Front Pharmacol. 2020 Sep 8;11:542902. doi: 10.3389/fphar.2020.542902. eCollection 2020.

DOI:10.3389/fphar.2020.542902
PMID:33013385
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7508177/
Abstract

This study aimed to explore the effects of 2,3,5,4'-tetrahydroxy-stilbene-2-O-β-d-glucoside (TSG) on the senescence of human umbilical vein cells (HUVEC) induced by hydrogen peroxide (HO) and to identify the potential targets mediating its protective action. HUVEC cells pre-treated with TSG for 24 h were exposed to HO treatment. TSG significantly decreased HO-induced cellular senescence, as indicated by reduced senescence-associated β-galactosidase (SA-β-gal) positive staining, the proportion of cells in the G1 phase, cell apoptosis, p21, and plasminogen activator inhibitor-1 (PAI-1) expression. Moreover, TSG promoted Sirtuin 1 (SIRT1) expression. When SIRT1 was inhibited by EX527 or SIRT1 siRNA, the effect of TSG is diminished according to the increased proportion of cells in the G1 phase, cell apoptosis, p21, and PAI-1 expression. Overall, our study established TSG as an anti-senescence compound that exerts its protective action by regulating SIRT1 expression.

摘要

本研究旨在探讨2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷（TSG）对过氧化氢（HO）诱导的人脐静脉细胞（HUVEC）衰老的影响，并确定介导其保护作用的潜在靶点。用TSG预处理24小时的HUVEC细胞接受HO处理。TSG显著降低了HO诱导的细胞衰老，表现为衰老相关β-半乳糖苷酶（SA-β-gal）阳性染色减少、G1期细胞比例降低、细胞凋亡减少、p21和纤溶酶原激活物抑制剂1（PAI-1）表达降低。此外，TSG促进了沉默调节蛋白1（SIRT1）的表达。当用EX527或SIRT1 siRNA抑制SIRT1时，根据G1期细胞比例增加、细胞凋亡增加、p21和PAI-1表达增加，TSG的作用减弱。总体而言，我们的研究确定TSG为一种抗衰老化合物，其通过调节SIRT1表达发挥保护作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf11/7508177/87ead6f8f504/fphar-11-542902-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf11/7508177/c03c3feddf0b/fphar-11-542902-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf11/7508177/35d57257bd1b/fphar-11-542902-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf11/7508177/86fc64ef976e/fphar-11-542902-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf11/7508177/60c97354afa3/fphar-11-542902-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf11/7508177/f01311c8b81a/fphar-11-542902-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf11/7508177/87ead6f8f504/fphar-11-542902-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf11/7508177/c03c3feddf0b/fphar-11-542902-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf11/7508177/35d57257bd1b/fphar-11-542902-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf11/7508177/86fc64ef976e/fphar-11-542902-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf11/7508177/60c97354afa3/fphar-11-542902-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf11/7508177/f01311c8b81a/fphar-11-542902-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf11/7508177/87ead6f8f504/fphar-11-542902-g006.jpg

相似文献

1
SIRT1 Is the Target Gene for 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-Glucoside Alleviating the HUVEC Senescence.SIRT1是2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷减轻人脐静脉内皮细胞衰老的靶基因。
Front Pharmacol. 2020 Sep 8;11:542902. doi: 10.3389/fphar.2020.542902. eCollection 2020.
2
Tetrahydroxystilbene glucoside alleviates angiotensin II induced HUVEC senescence via SIRT1.四羟基二苯乙烯葡萄糖苷通过 SIRT1 减轻血管紧张素 II 诱导的 HUVEC 衰老。
Can J Physiol Pharmacol. 2021 Apr;99(4):389-394. doi: 10.1139/cjpp-2020-0202. Epub 2020 Sep 8.
3
Tetrahydroxy stilbene glycoside attenuates endothelial cell premature senescence induced by HO through the microRNA-34a/SIRT1 pathway.四羟基二苯乙烯苷通过 microRNA-34a/SIRT1 通路减轻 HO 诱导的内皮细胞过早衰老。
Sci Rep. 2022 Feb 1;12(1):1708. doi: 10.1038/s41598-022-05804-9.
4
Ginsenoside Rb1 prevents H2O2-induced HUVEC senescence by stimulating sirtuin-1 pathway.人参皂苷 Rb1 通过刺激沉默调节蛋白-1 通路来防止 H2O2 诱导的 HUVEC 衰老。
PLoS One. 2014 Nov 11;9(11):e112699. doi: 10.1371/journal.pone.0112699. eCollection 2014.
5
[Tanshinone IIA attenuates hydrogen peroxide-induced senescence of human umbilical vein endothelial cells through activating SIRT1/eNOS pathway].丹参酮IIA通过激活SIRT1/eNOS通路减轻过氧化氢诱导的人脐静脉内皮细胞衰老
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2019 Sep;35(9):806-811.
6
Sirt1 modulates premature senescence-like phenotype in human endothelial cells.沉默调节蛋白1调控人内皮细胞的早衰样表型。
J Mol Cell Cardiol. 2007 Nov;43(5):571-9. doi: 10.1016/j.yjmcc.2007.08.008. Epub 2007 Aug 22.
7
Total flavonoids from the Carya cathayensis Sarg. leaves inhibit HUVEC senescence through the miR-34a/SIRT1 pathway.山核桃叶总黄酮通过 miR-34a/SIRT1 通路抑制 HUVEC 衰老。
J Cell Biochem. 2019 Oct;120(10):17240-17249. doi: 10.1002/jcb.28986. Epub 2019 May 20.
8
Effects of Rhein lysinate on H2O2-induced cellular senescence of human umbilical vascular endothelial cells.赖氨匹林对 H2O2 诱导的人脐静脉内皮细胞衰老的影响。
Acta Pharmacol Sin. 2011 Oct;32(10):1246-52. doi: 10.1038/aps.2011.101. Epub 2011 Sep 12.
9
Curcumin Attenuates Hydrogen Peroxide-Induced Premature Senescence via the Activation of SIRT1 in Human Umbilical Vein Endothelial Cells.姜黄素通过激活人脐静脉内皮细胞中的SIRT1减轻过氧化氢诱导的早衰。
Biol Pharm Bull. 2015;38(8):1134-41. doi: 10.1248/bpb.b15-00012.
10
Involvement of Flavonoids from the Leaves of Sarg. in Sirtuin 1 Expression in HUVEC Senescence.羊栖菜叶片中黄酮类化合物对人脐静脉内皮细胞衰老过程中沉默信息调节因子1表达的影响
Evid Based Complement Alternat Med. 2018 Jul 8;2018:8246560. doi: 10.1155/2018/8246560. eCollection 2018.

引用本文的文献

1
Nutrition Interventions of Herbal Compounds on Cellular Senescence.草药化合物对细胞衰老的营养干预。
Oxid Med Cell Longev. 2022 Apr 27;2022:1059257. doi: 10.1155/2022/1059257. eCollection 2022.
2
Tetrahydroxy stilbene glycoside attenuates endothelial cell premature senescence induced by HO through the microRNA-34a/SIRT1 pathway.四羟基二苯乙烯苷通过 microRNA-34a/SIRT1 通路减轻 HO 诱导的内皮细胞过早衰老。
Sci Rep. 2022 Feb 1;12(1):1708. doi: 10.1038/s41598-022-05804-9.
3
Apremilast prevents IL‑17‑induced cellular senescence in ATDC5 chondrocytes mediated by SIRT1.

本文引用的文献

1
Tumor Necrosis Factor-alpha (TNF-α) Enhances miR-155-Mediated Endothelial Senescence by Targeting Sirtuin1 (SIRT1).肿瘤坏死因子-α（TNF-α）通过靶向沉默调节蛋白 1（SIRT1）增强 miR-155 介导的血管内皮衰老。
Med Sci Monit. 2019 Nov 21;25:8820-8835. doi: 10.12659/MSM.919721.
2
2,3,5,4'-Tetrahydroxystilbene-2-O-β-glucoside potentiates self-renewal of human dental pulp stem cells via the AMPK/ERK/SIRT1 axis.2,3,5,4'-四羟基二苯乙烯-2-O-β-葡萄糖苷通过 AMPK/ERK/SIRT1 轴增强人牙髓干细胞的自我更新。
Int Endod J. 2018 Oct;51(10):1159-1170. doi: 10.1111/iej.12935. Epub 2018 Apr 26.
3
MiR-34a modulates ionizing radiation-induced senescence in lung cancer cells.
阿普米司特通过 SIRT1 防止 ATDC5 软骨细胞中由 IL-17 诱导的细胞衰老。
Int J Mol Med. 2021 Mar;47(3). doi: 10.3892/ijmm.2021.4845. Epub 2021 Jan 15.
微小RNA-34a调节电离辐射诱导的肺癌细胞衰老。
Oncotarget. 2017 Jul 15;8(41):69797-69807. doi: 10.18632/oncotarget.19267. eCollection 2017 Sep 19.
4
Tetrahydroxy stilbene glucoside ameliorates H2O2-induced human brain microvascular endothelial cell dysfunction in vitro by inhibiting oxidative stress and inflammatory responses.四羟基二苯乙烯葡萄糖苷通过抑制氧化应激和炎症反应改善 H2O2 诱导的体外人脑血管内皮细胞功能障碍。
Mol Med Rep. 2017 Oct;16(4):5219-5224. doi: 10.3892/mmr.2017.7225. Epub 2017 Aug 10.
5
Cellular Senescence: What, Why, and How.细胞衰老：是什么、为何以及如何发生。
Wounds. 2017 Jun;29(6):168-174.
6
Plasminogen Activator Inhibitor-1 Is a Marker and a Mediator of Senescence.纤溶酶原激活物抑制剂-1是衰老的一个标志物和介质。
Arterioscler Thromb Vasc Biol. 2017 Aug;37(8):1446-1452. doi: 10.1161/ATVBAHA.117.309451. Epub 2017 Jun 1.
7
Inhibition of Hydrogen Peroxide-Induced Human Umbilical Vein Endothelial Cells Aging by Allicin Depends on Sirtuin1 Activation.大蒜素通过激活沉默调节蛋白1抑制过氧化氢诱导的人脐静脉内皮细胞衰老
Med Sci Monit. 2017 Jan 31;23:563-570. doi: 10.12659/msm.899730.
8
2,3,5,4'-Tetrahydroxystilbene-2-O--D-glucoside Promotes Expression of the Longevity Gene Klotho.2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷促进长寿基因α-klotho的表达。
Oxid Med Cell Longev. 2016;2016:3128235. doi: 10.1155/2016/3128235. Epub 2016 Nov 3.
9
Cellular Model of p21-Induced Senescence.p21诱导衰老的细胞模型。
Methods Mol Biol. 2017;1534:31-39. doi: 10.1007/978-1-4939-6670-7_3.
10
Is senescence-associated β-galactosidase a marker of neuronal senescence?衰老相关β-半乳糖苷酶是神经元衰老的标志物吗？
Oncotarget. 2016 Dec 6;7(49):81099-81109. doi: 10.18632/oncotarget.12752.