Zhang Jinping, Zhong Yongzhi, Tang Rui, Rebijith K B, Li Fengqi, Chen Guohua, Zhang Feng
MARA-CABI Joint Laboratory for Bio-safety, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
College of Plant Protection, Yunnan Agricultural University, Kunming, China.
Front Physiol. 2020 Sep 3;11:1058. doi: 10.3389/fphys.2020.01058. eCollection 2020.
The samurai wasp, , is the predominant egg parasitoid of the brown marmorated stink bug, , in its native range in China. ()-2-Decenal is a major component of the alarm pheromone of , an important invasive insect pest with significant economic importance. . can be strongly repelled by ()-2-decenal, and thus its host location efficiency would be reduced in the field. Better understanding on the molecular basis of olfactory reception of this host alarm pheromone component by may provide opportunities to develop novel approaches to enhance biological control efficacy of the parasitoid against . We identified six Odorant Binding Proteins (OBPs) from by transcriptome sequencing, within which three classical OBPs were expressed in a heterologous expression system with , harvested, and then challenged with ()-2-decenal in binding assay experiments. OBP2 showed the highest binding ability to ()-2-decenal, compared to OBP1 and OBP3. Our results unambiguously suggest that OBP2 would play an important role in discriminating ()-2-decenal and could be a possible target for further intervention in the parasitoid-host system.
武士黄蜂是中国本土范围内褐飞蝽的主要卵寄生蜂。（）-2-癸烯醛是褐飞蝽警报信息素的主要成分，褐飞蝽是一种具有重要经济意义的重要入侵害虫。武士黄蜂会被（）-2-癸烯醛强烈排斥，因此其在野外的寄主定位效率会降低。更好地了解武士黄蜂对这种寄主警报信息素成分的嗅觉接收分子基础，可能会为开发新方法以提高寄生蜂对褐飞蝽的生物防治效果提供机会。我们通过转录组测序从武士黄蜂中鉴定出六种气味结合蛋白（OBP），其中三种经典OBP在与[具体内容缺失]的异源表达系统中表达、收获，然后在结合测定实验中用（）-2-癸烯醛进行挑战。与OBP1和OBP3相比，OBP2对（）-2-癸烯醛的结合能力最高。我们的结果明确表明，OBP2在识别（）-2-癸烯醛方面将发挥重要作用，并且可能是进一步干预寄生蜂 - 寄主系统的一个潜在靶点。
