Wang Zehua, Yang Fan, Sun Ang, Song Jingyi, Shan Shuang, Zhang Yongjun, Wang Shanning
Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Int J Biol Macromol. 2022 May 1;206:759-767. doi: 10.1016/j.ijbiomac.2022.03.084. Epub 2022 Mar 17.
Odorant-binding proteins (OBPs) play essential roles in the functioning of insect peripheral olfactory systems. To fully understand the olfactory roles of OBPs in Halyomorpha halys, an important invasive pest found worldwide, we studied the expression and functional characterization of five OBP-associated genes from H. halys that are clustered in the genome. The tissue distribution of the OBP gene cluster suggests that these genes were enriched in nymph and adult antennae, indicating their possible involvement in the chemosensory process. The different expression levels of the five OBPs in nymph and adult antennae suggest that this gene cluster is regulated independently. Ligand-binding experiments have shown similar specificities of these five OBPs towards several organic compounds, including the alarm pheromone of H. halys (trans-2-decenal), the aggregation pheromone of Plautia stali (methyl (2E, 4E, 6Z)-decatrienoate), and plant volatile compounds (e.g., cis-3-hexenyl benzoate and β-ionone). In particular, trans-2-dodecenal, an alarm pheromone analog, exhibited high affinity to the five OBP proteins and alarm pheromone activity towards H. halys. Thus, this OBP cluster may mediate the response of stink bugs to the both the alarm pheromone and host-related volatiles and could be an interesting target to design novel olfactory regulators for the management of H. halys infestations.
气味结合蛋白（OBPs）在昆虫外周嗅觉系统的功能中发挥着重要作用。为了全面了解OBPs在全球范围内发现的重要入侵害虫——褐飞虱中的嗅觉作用，我们研究了褐飞虱基因组中五个成簇的OBP相关基因的表达和功能特性。OBP基因簇的组织分布表明，这些基因在若虫和成虫触角中富集，表明它们可能参与了化学感受过程。五个OBPs在若虫和成虫触角中的不同表达水平表明该基因簇是独立调控的。配体结合实验表明，这五个OBPs对几种有机化合物具有相似的特异性，包括褐飞虱的报警信息素（反式-2-癸烯醛）、斯氏绿蝽的聚集信息素（(2E, 4E, 6Z)-癸三烯酸甲酯）和植物挥发性化合物（如顺式-3-己烯基苯甲酸酯和β-紫罗兰酮）。特别是，报警信息素类似物反式-2-十二碳烯醛对这五个OBP蛋白表现出高亲和力，并对褐飞虱具有报警信息素活性。因此，这个OBP簇可能介导了椿象对报警信息素和宿主相关挥发物的反应，并且可能是设计新型嗅觉调节剂以管理褐飞虱虫害的一个有趣靶点。
