• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

D-氨基酸混合物增强CMIT/MIT对腐蚀性生物膜的杀菌效果。

A Mixture of D-Amino Acids Enhances the Biocidal Efficacy of CMIT/MIT Against Corrosive Biofilm.

作者信息

Liu Xiaomeng, Li Zhong, Fan Yongqiang, Lekbach Yassir, Song Yongbo, Xu Dake, Zhang Zhichao, Ding Lei, Wang Fuhui

机构信息

School of Life Sciences and Biopharmaceuticals, Shenyang Pharmaceutical University, Shenyang, China.

College of Life and Health Sciences, Northeastern University, Shenyang, China.

出版信息

Front Microbiol. 2020 Sep 4;11:557435. doi: 10.3389/fmicb.2020.557435. eCollection 2020.

DOI:10.3389/fmicb.2020.557435
PMID:33013788
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7498826/
Abstract

Biocides are widely used for the mitigation of microbial contamination, especially in the field of the aviation fuel industry. However, the long-term use of biocide has raised the concerns regarding the environmental contamination and microbial drug resistance. In this study, the effect of a mixture of D-amino acids (D-tyrosine and D-methionine) on the enhancement of the bactericidal effect of 5-Chloro-2-Methyl-4-isothiazolin-3-one/2-Methyl-2H-isothiazole-3-one (CMIT/MIT) against corrosive biofilm was evaluated. The results revealed that D-Tyr and D-Met alone can enhance the biocidal efficacy of CMIT/MIT, while the treatment of 5 ppm CMIT/MIT, 1 ppm D-Tyr and 100 ppm D-Met showed the best efficacy comparable to that of 25 ppm CMIT/MIT alone. The triple combination treatment successfully prevented the establishment of the corrosive biofilm and effectively removed the mature biofilm. These conclusions were confirmed by the results of sessile cell counts, images obtained by scanning electron microscope and confocal laser scanning microscope, and the ATP test kit.

摘要

杀生剂被广泛用于减轻微生物污染，尤其是在航空燃料工业领域。然而，长期使用杀生剂引发了对环境污染和微生物耐药性的担忧。在本研究中，评估了D-氨基酸（D-酪氨酸和D-甲硫氨酸）混合物对增强5-氯-2-甲基-4-异噻唑啉-3-酮/2-甲基-2H-异噻唑啉-3-酮（CMIT/MIT）对腐蚀性生物膜杀菌效果的影响。结果表明，单独的D-酪氨酸和D-甲硫氨酸可增强CMIT/MIT的杀菌效力，而5 ppm CMIT/MIT、1 ppm D-酪氨酸和100 ppm D-甲硫氨酸的处理显示出与单独使用25 ppm CMIT/MIT相当的最佳效果。三联组合处理成功防止了腐蚀性生物膜的形成，并有效去除了成熟生物膜。这些结论通过固着细胞计数结果、扫描电子显微镜和共聚焦激光扫描显微镜获得的图像以及ATP检测试剂盒得到了证实。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/fff8bc46a330/fmicb-11-557435-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/a833cb5e57bf/fmicb-11-557435-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/b5c0c7598527/fmicb-11-557435-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/b5c08377303b/fmicb-11-557435-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/6db1d28872dd/fmicb-11-557435-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/b4ec416ca149/fmicb-11-557435-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/3386174c3529/fmicb-11-557435-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/e57b4c205da7/fmicb-11-557435-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/2dc2f0c11f82/fmicb-11-557435-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/5382f6bb83d8/fmicb-11-557435-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/d39e2937b47b/fmicb-11-557435-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/258d0702f7f4/fmicb-11-557435-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/fff8bc46a330/fmicb-11-557435-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/a833cb5e57bf/fmicb-11-557435-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/b5c0c7598527/fmicb-11-557435-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/b5c08377303b/fmicb-11-557435-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/6db1d28872dd/fmicb-11-557435-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/b4ec416ca149/fmicb-11-557435-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/3386174c3529/fmicb-11-557435-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/e57b4c205da7/fmicb-11-557435-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/2dc2f0c11f82/fmicb-11-557435-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/5382f6bb83d8/fmicb-11-557435-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/d39e2937b47b/fmicb-11-557435-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/258d0702f7f4/fmicb-11-557435-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/fff8bc46a330/fmicb-11-557435-g012.jpg

相似文献

1
A Mixture of D-Amino Acids Enhances the Biocidal Efficacy of CMIT/MIT Against Corrosive Biofilm.D-氨基酸混合物增强CMIT/MIT对腐蚀性生物膜的杀菌效果。
Front Microbiol. 2020 Sep 4;11:557435. doi: 10.3389/fmicb.2020.557435. eCollection 2020.
2
Degradation of a non-oxidizing biocide in circulating cooling water using UV/persulfate: Kinetics, pathways, and cytotoxicity.循环冷却水中使用 UV/过硫酸盐非氧化杀生剂的降解：动力学、途径和细胞毒性。
Chemosphere. 2022 Feb;289:133064. doi: 10.1016/j.chemosphere.2021.133064. Epub 2021 Nov 24.
3
Insights into the mechanisms of within-species variation in sensitivity to chemicals: A case study using daphnids exposed to CMIT/MIT biocide.探讨物种内对化学品敏感性变化的机制：以三氯生/甲基异噻唑啉酮杀生剂暴露下的水蚤为例。
Ecotoxicol Environ Saf. 2023 Jun 15;258:114967. doi: 10.1016/j.ecoenv.2023.114967. Epub 2023 May 9.
4
Experimental determination of indoor air concentration of 5-chloro-2-methylisothiazol-3(2H)-one/ 2-methylisothiazol-3(2H)-one (CMIT/MIT) emitted by the use of humidifier disinfectant.通过使用加湿器消毒剂对室内空气中5-氯-2-甲基异噻唑-3(2H)-酮/2-甲基异噻唑-3(2H)-酮（CMIT/MIT）浓度的实验测定。
Environ Anal Health Toxicol. 2020 Jun;35(2):e2020008. doi: 10.5620/eaht.e2020008. Epub 2020 Jun 30.
5
Effects of 5-chloro-2-methyl-4-isothiazolin-3-one and other candidate biodiesel biocides on rat alveolar macrophages and NR8383 cells.5-氯-2-甲基-4-异噻唑啉-3-酮和其他候选生物柴油杀菌剂对大鼠肺泡巨噬细胞和 NR8383 细胞的影响。
Arch Toxicol. 2011 Nov;85(11):1419-27. doi: 10.1007/s00204-011-0689-y. Epub 2011 Mar 29.
6
Enhanced Biocide Treatments with D-amino Acid Mixtures against a Biofilm Consortium from a Water Cooling Tower.用D-氨基酸混合物强化杀生剂处理以对抗来自水冷却塔的生物膜群落
Front Microbiol. 2017 Aug 16;8:1538. doi: 10.3389/fmicb.2017.01538. eCollection 2017.
7
Enhanced Biocide Mitigation of Field Biofilm Consortia by a Mixture of D-Amino Acids.D-氨基酸混合物对现场生物膜群落生物杀灭作用的增强
Front Microbiol. 2016 Jun 13;7:896. doi: 10.3389/fmicb.2016.00896. eCollection 2016.
8
Functional and dynamic mitochondrial damage by chloromethylisothiazolinone/methylisothiazolinone (CMIT/MIT) mixture in brain endothelial cell lines and rat cerebrovascular endothelium.氯甲基异噻唑啉酮/甲基异噻唑啉酮（CMIT/MIT）混合物对脑内皮细胞系和大鼠脑血管内皮细胞的功能和动态线粒体损伤。
Toxicol Lett. 2022 Aug 1;366:45-57. doi: 10.1016/j.toxlet.2022.06.010. Epub 2022 Jul 5.
9
Trans- and Multigenerational Effects of Isothiazolinone Biocide CMIT/MIT on Genotoxicity and Epigenotoxicity in .异噻唑啉酮杀菌剂CMIT/MIT对……的遗传毒性和表观遗传毒性的跨代及多代效应
Toxics. 2023 Apr 20;11(4):388. doi: 10.3390/toxics11040388.
10
Mechanism of 5-chloro-2-methyl-4-isothiazolin-3-one (CMIT) in controlling microbial problems in aircraft fuel systems.5-氯-2-甲基-4-异噻唑啉-3-酮（CMIT）控制飞机燃油系统微生物问题的机制。
RSC Adv. 2023 Jun 28;13(28):19485-19494. doi: 10.1039/d3ra02970k. eCollection 2023 Jun 22.

引用本文的文献

1
d-tyrosine enhances disoctyl dimethyl ammonium chloride on alleviating SRB corrosion.D-酪氨酸增强二辛基二甲基氯化铵对缓解SRB腐蚀的作用。
Heliyon. 2023 Oct 28;9(11):e21755. doi: 10.1016/j.heliyon.2023.e21755. eCollection 2023 Nov.
2
Targeted Anti-Biofilm Therapy: Dissecting Targets in the Biofilm Life Cycle.靶向抗生物膜治疗：剖析生物膜生命周期中的靶点。
Pharmaceuticals (Basel). 2022 Oct 12;15(10):1253. doi: 10.3390/ph15101253.

本文引用的文献

1
Application of D-Amino Acids as Biofilm Dispersing Agent in Dental Unit Waterlines.D-氨基酸作为牙科设备水线生物膜分散剂的应用。
Int J Dent. 2018 Jan 14;2018:9413925. doi: 10.1155/2018/9413925. eCollection 2018.
2
Mitigation of a nitrate reducing Pseudomonas aeruginosa biofilm and anaerobic biocorrosion using ciprofloxacin enhanced by D-tyrosine.利用 D-酪氨酸增强环丙沙星对硝酸还原假单胞菌生物膜和厌氧生物腐蚀的缓解作用。
Sci Rep. 2017 Jul 31;7(1):6946. doi: 10.1038/s41598-017-07312-7.
3
Understanding the Mechanism of Bacterial Biofilms Resistance to Antimicrobial Agents.
了解细菌生物膜对抗菌剂的耐药机制。
Open Microbiol J. 2017 Apr 28;11:53-62. doi: 10.2174/1874285801711010053. eCollection 2017.
4
Enhanced Biocide Mitigation of Field Biofilm Consortia by a Mixture of D-Amino Acids.D-氨基酸混合物对现场生物膜群落生物杀灭作用的增强
Front Microbiol. 2016 Jun 13;7:896. doi: 10.3389/fmicb.2016.00896. eCollection 2016.
5
Mechanistic modeling of biocorrosion caused by biofilms of sulfate reducing bacteria and acid producing bacteria.由硫酸盐还原菌和产酸菌生物膜引起的生物腐蚀的机理建模
Bioelectrochemistry. 2016 Aug;110:52-8. doi: 10.1016/j.bioelechem.2016.03.003. Epub 2016 Mar 24.
6
D-Amino acids inhibit biofilm formation in Staphylococcus epidermidis strains from ocular infections.D-氨基酸可抑制眼部感染表皮葡萄球菌菌株中的生物膜形成。
J Med Microbiol. 2014 Oct;63(Pt 10):1369-1376. doi: 10.1099/jmm.0.075796-0. Epub 2014 Jul 7.
7
D-amino acids enhance the activity of antimicrobials against biofilms of clinical wound isolates of Staphylococcus aureus and Pseudomonas aeruginosa.D-氨基酸可增强抗菌药物对金黄色葡萄球菌和铜绿假单胞菌临床伤口分离株生物膜的活性。
Antimicrob Agents Chemother. 2014 Aug;58(8):4353-61. doi: 10.1128/AAC.02468-14. Epub 2014 May 19.
8
Biofilm formation and persistence on abiotic surfaces in the context of food and medical environments.生物膜在食品和医疗环境中于非生物表面的形成和持续存在。
Arch Microbiol. 2014 Jul;196(7):453-72. doi: 10.1007/s00203-014-0983-1. Epub 2014 Apr 18.
9
Current and emerging environmentally-friendly systems for fouling control in the marine environment.当前和新兴的海洋环境中防污的环保系统。
Biotechnol Adv. 2013 Dec;31(8):1738-53. doi: 10.1016/j.biotechadv.2013.09.002. Epub 2013 Sep 16.
10
The extracellular matrix Component Psl provides fast-acting antibiotic defense in Pseudomonas aeruginosa biofilms.细胞外基质成分 Psl 为铜绿假单胞菌生物膜提供快速作用的抗生素防御。
PLoS Pathog. 2013;9(8):e1003526. doi: 10.1371/journal.ppat.1003526. Epub 2013 Aug 8.