Popov Boris, Petrov Nikolai, Ryabov Vladimir, Evsyukov Igor
Institute of Cytology Russian Academy of Sciences, St. Petersburg 194064, Russia.
Children's Hospital, The Ohio State University, Columbus, OH 43205-2696, USA.
Stem Cells Int. 2020 Sep 18;2020:8883436. doi: 10.1155/2020/8883436. eCollection 2020.
An effective regulation of quiescence plays a key role in the differentiation, plasticity, and prevention of stem cells from becoming malignant. The state of quiescence is being controlled by the pRb family proteins which show overlapping functions in cell cycle regulation; however, their roles in controlling the proliferation of mesenchymal stem cells (MSCs) remain to be understood. This study investigated the regulation of transient quiescence using growth curves, proliferation assay, the cytometric evaluation of cell cycle, Western blotting, and the electromobility gel shift assay (EMSA) on synchronized MSCs of the C3H10Т1/2 and control cells with different statuses of pRb proteins. It has been found that functional steady-state level of p130 but not pRb plays a critical role for entering, exiting, and maintenance of transient quiescence in multipotent mesenchymal stem cells.
静止状态的有效调节在干细胞的分化、可塑性以及防止其恶变方面起着关键作用。静止状态受pRb家族蛋白控制，这些蛋白在细胞周期调控中具有重叠功能；然而，它们在控制间充质干细胞（MSC）增殖方面的作用仍有待了解。本研究使用生长曲线、增殖测定、细胞周期的细胞计量学评估、蛋白质免疫印迹法以及对具有不同pRb蛋白状态的C3H10Т1/2同步化间充质干细胞和对照细胞进行的电泳迁移率凝胶位移分析（EMSA），研究了短暂静止的调节。研究发现，对于多能间充质干细胞进入、退出和维持短暂静止状态，起关键作用的是p130而非pRb的功能性稳态水平。
