School of Medicine & Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
Department of Oncology, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, China.
Dis Markers. 2020 Sep 15;2020:3461315. doi: 10.1155/2020/3461315. eCollection 2020.
Although intestinal microbial dysbiosis was confirmed to be associated with many chronic diseases and health status through complicated interaction with the host, the effect on gastric cancer was less studied. In this study, we sequenced the 16S rRNA and 18S rRNA genes of fecal bacteria and fungi, respectively, in 134 gastric cancer patients and 58 healthy controls matched by age and gender. Propensity score matching (PSM) was adopted for adjusting diet habits and lifestyle, and 44 patients and 44 healthy controls (matching population) were enrolled. Serum antibody to and metabolites of the matching population were detected. The positive rates of antibody to between the patients and the control group did not reach the statistical difference. LEfSe analysis indicated that bacteria were more stable than fungi when adjusting diet and lifestyle. , , and genus and subsp. , , and species in bacteria were related to the risk of gastric cancer and showed a good diagnostic value in distinguishing the patients from healthy controls. showed a risk effect for gastric cancer; however, the effect turned into be protective after PSM. Serum L-alanine, L-threonine, and methionol were positively associated with and and several fungi genus. Overall, our findings indicated that fecal microbiome constitution alteration may be associated with gastric cancer through influencing the amino acid metabolism.
尽管肠道微生物失调通过与宿主的复杂相互作用被证实与许多慢性疾病和健康状况有关，但对胃癌的影响研究较少。在这项研究中，我们分别对 134 名胃癌患者和 58 名年龄和性别相匹配的健康对照者的粪便细菌和真菌 16S rRNA 和 18S rRNA 基因进行了测序。采用倾向评分匹配（PSM）调整饮食习惯和生活方式，纳入了 44 名患者和 44 名健康对照者（匹配人群）。检测了匹配人群的血清抗体和代谢物。患者组和对照组之间抗体阳性率未达到统计学差异。LEfSe 分析表明，在调整饮食和生活方式后，细菌比真菌更稳定。细菌中的 、 、 和 属以及 亚属 、 、 和 种与胃癌风险相关，在区分患者和健康对照者方面具有良好的诊断价值。 对胃癌有风险作用；然而，PSM 后作用变为保护作用。血清 L-丙氨酸、L-苏氨酸和甲硫醇与 和几种真菌属呈正相关。总的来说，我们的研究结果表明，粪便微生物组成的改变可能通过影响氨基酸代谢与胃癌有关。
