Qiu Tingting, Yang Jinge, Pan Teng, Peng Chihan, Jiang Huabei, Luo Yan
Department of Medical Ultrasound, Sichuan University West China Hospital, Chengdu, China.
School of Optoelectric Engineering, Chongqing University of Posts and Telecommunications, Chongqing 400065, China.
Biomed Opt Express. 2020 Jun 25;11(7):3985-3995. doi: 10.1364/BOE.394344. eCollection 2020 Jul 1.
Assessment of liver function reserve (LFR) is essential to determine liver resection scope and predict prognosis for patients with liver disease. Indocyanine green (ICG) concentration change is a classic marker to reflect liver function reserve as ICG is selectively taken up and eliminated by liver. Here we proposed a noninvasive approach for LFR assessment based on a real-time photoacoustic tomography (PAT) system. This feasibility study was to detect ICG concentration change by PAT in phantom and in vivo using both normal and partial hepatectomy (PH) rabbits. A linear relationship between photoacoustic signal intensity of ICG and ICG concentration was found in vitro. In vivo ICG concentration change over time after ICG injection was observed by PAT in normal rabbits, which was consistent with the findings measured by invasive spectrophotometry. Finally, clear difference in ICG clearance between the control and PH models was identified by PAT. Taken together, our study indicated the clinical potential of PAT to in vivo evaluate LFR noninvasively.
评估肝功能储备（LFR）对于确定肝病患者的肝切除范围和预测预后至关重要。吲哚菁绿（ICG）浓度变化是反映肝功能储备的经典标志物，因为ICG被肝脏选择性摄取和清除。在此，我们基于实时光声断层扫描（PAT）系统提出了一种用于LFR评估的非侵入性方法。这项可行性研究旨在使用正常和部分肝切除术（PH）兔子，通过PAT在体模和体内检测ICG浓度变化。在体外发现ICG的光声信号强度与ICG浓度之间存在线性关系。通过PAT在正常兔子中观察到ICG注射后体内ICG浓度随时间的变化，这与侵入性分光光度法测量的结果一致。最后，通过PAT确定了对照组和PH模型之间ICG清除率的明显差异。综上所述，我们的研究表明PAT在体内无创评估LFR方面具有临床潜力。
