Bueno Juan M, Cruz-Castillo Ricardo, Avilés-Trigueros Marcelino, Bautista-Elivar Nazario
Laboratorio de Óptica, Instituto Universitario de Investigación en Óptica y Nanofísica, Universidad de Murcia, Murcia, Spain.
Área Académica de Matemáticas y Física, Instituto de Ciencias Básicas e Ingeniería, Universidad Autónoma del Estado de Hidalgo, Hidalgo, Mexico.
Biomed Opt Express. 2020 Aug 7;11(9):4901-4914. doi: 10.1364/BOE.399835. eCollection 2020 Sep 1.
Diabetic retinopathy (DR) is defined as a microvascular pathology. However, some data have suggested that the retinal photoreceptors (PRs) might be important in the pathogenesis of this ocular disease. In this study the organization of the PRs in control and diabetic-induced rats was compared using multiphoton microscopy. The PR mosaic was imaged at different locations in non-stained retinas. The density of PRs was directly quantified from cell counting. The spatially resolved density presents a double-slope pattern (from the central retina towards the periphery) in both healthy and pathological samples, although the values for the latter were significantly lower all across the retina. Moreover, Voronoi analysis was performed to explore changes in PR topography. In control specimens a hexagonally packed structure was dominant. However, despite the non-controlled effects of the disease in retinal structures, this PR regularity was fairly maintained in diabetic retinas.
糖尿病性视网膜病变（DR）被定义为一种微血管病变。然而，一些数据表明视网膜光感受器（PRs）可能在这种眼部疾病的发病机制中起重要作用。在本研究中，使用多光子显微镜比较了对照大鼠和糖尿病诱导大鼠中PRs的组织结构。在未染色的视网膜的不同位置对PR镶嵌进行成像。通过细胞计数直接定量PRs的密度。在健康和病理样本中，空间分辨密度均呈现双斜率模式（从视网膜中央向周边），尽管后者在整个视网膜中的值均显著较低。此外，进行了Voronoi分析以探索PR地形的变化。在对照标本中，六边形堆积结构占主导地位。然而，尽管疾病对视网膜结构有不可控的影响，但这种PR规则性在糖尿病视网膜中仍相当保持。
