Hiramatsu K, Nozaki H, Arimori S
Diabetologia. 1987 May;30(5):310-3. doi: 10.1007/BF00299023.
To examine the effect of serum insulin independent of the level of blood glucose in vivo on platelet aggregation in healthy individuals, a euglycaemic insulin clamp was applied up to 4 h. During the clamp, blood glucose at 5.0 mmol/l and insulin levels at 100 microU/ml were maintained. Blood samples were drawn before, 2 and 4 h after the start of the insulin clamp. The platelet aggregation induced by 1 mumol/l and 2 mumol/l ADP, 1 microgram/ml collagen and 2.7 mumol/l epinephrine was measured in the blood samples. Platelet aggregation induced by adenosine diphosphate, collagen and epinephrine in the 4 h sample was significantly reduced from the pre-clamp value of 8.4% to 3.9% (p less than 0.05), 26.2% to 7.0% (p less than 0.01) and 31.8% to 9.1% (p less than 0.01), respectively. On the other hand, when the same individuals were infused with physiological saline and blood glucose (4.4 mmol/l) and insulin level (10 mIU/l) were kept within normal values, there was no difference between the values of induced platelet aggregation in samples drawn before and during the insulin infusion. It was concluded that hyperinsulinaemia reduces platelet aggregation in vivo when euglycaemia was maintained.
为了研究在体内血糖水平正常情况下血清胰岛素对健康个体血小板聚集的影响，应用正常血糖胰岛素钳夹技术长达4小时。在钳夹过程中，将血糖维持在5.0 mmol/l，胰岛素水平维持在100微单位/毫升。在胰岛素钳夹开始前、开始后2小时和4小时采集血样。测定血样中由1微摩尔/升和2微摩尔/升二磷酸腺苷（ADP）、1微克/毫升胶原和2.7微摩尔/升肾上腺素诱导的血小板聚集。4小时样本中由二磷酸腺苷、胶原和肾上腺素诱导的血小板聚集分别从钳夹前的值8.4%显著降低至3.9%（p<0.05）、26.2%至7.0%（p<0.01）和31.8%至9.1%（p<0.01）。另一方面，当给同一批个体输注生理盐水并将血糖（4.4 mmol/l）和胰岛素水平（10 mIU/l）维持在正常范围内时，胰岛素输注前和输注期间采集的样本中诱导的血小板聚集值没有差异。得出的结论是，在维持正常血糖的情况下，高胰岛素血症会降低体内血小板聚集。
