Targonska Sara, Rewak-Soroczynska Justyna, Piecuch Agata, Paluch Emil, Szymanski Damian, Wiglusz Rafal J
Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, 50-422 Wroclaw, Poland.
Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland.
ACS Omega. 2020 Sep 15;5(38):24546-24557. doi: 10.1021/acsomega.0c03044. eCollection 2020 Sep 29.
New polymer-inorganic composites with antibiofilm features based on the granulated poly(tetrafluoroethylene) (PTFE) and apatite materials were obtained using a standard hydraulic press. The study was performed in hydroxy- and fluorapatites doped with different amounts of silver ions and followed by heat treatment at 600 °C. The structural, morphological, and physicochemical properties were determined by X-ray powder diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy-energy-dispersive spectrometry (SEM-EDS), and transition electron microscopy (TEM). The antibacterial properties of the obtained materials were evaluated against Gram-negative pathogens such as , , and as well as against Gram-positive bacteria . The cytotoxicity assessment was carried out on the red blood cells (RBC) as a cell model for in vitro study. Moreover, the biofilm formation on the biocomposite surface was studied using confocal laser scanning microscopy (CLSM).
基于粒状聚四氟乙烯（PTFE）和磷灰石材料，利用标准液压机获得了具有抗生物膜特性的新型聚合物-无机复合材料。该研究在掺杂不同量银离子的羟基磷灰石和氟磷灰石中进行，随后在600℃下进行热处理。通过X射线粉末衍射（XRD）、傅里叶变换红外（FT-IR）光谱、扫描电子显微镜-能量色散光谱（SEM-EDS）和透射电子显微镜（TEM）测定结构、形态和物理化学性质。评估所得材料对革兰氏阴性病原体如 、 和 以及革兰氏阳性菌 的抗菌性能。以红细胞（RBC）作为细胞模型进行体外细胞毒性评估。此外，使用共聚焦激光扫描显微镜（CLSM）研究生物复合材料表面的生物膜形成。
