School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian 223003, China.
School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai 200240, China.
Int J Environ Res Public Health. 2019 Feb 19;16(4):598. doi: 10.3390/ijerph16040598.
In the 21st century, with ever-increasing consciousness and social awareness, researchers must tackle the microbial infections that pose a major threat to human safety. For many reasons, the emergence/re-emergence of threatening pathogens has increased and poses a serious challenge to health care services. Considering the changing dynamics of 21st-century materials with medical potentialities, the integration of bioactive agents into materials to engineer antibacterial matrices has received limited attention so far. Thus, antimicrobial active conjugates are considered potential candidates to eradicate infections and reduce microbial contaminations in healthcare facilities. In this context, eco-friendly and novel conjugates with antimicrobial, antibiofilm, and anticancer potentialities were developed using biogenic silver nanoparticles (AgNPs) from () extract and chitosan (CHI). A range of instrumental and imaging tools, i.e., UV-Vis and FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD), were employed to characterize the freshly extracted AgNPs. Biogenic AgNPs obtained after a 24-h reaction period were used to engineer CHI-based conjugates and designated as CHI‒AgNPs1 to CHI‒AgNPs5, subject to the AgNPs concentration. After the stipulated loading period, 92% loading efficiency (LE) was recorded for a CHI‒AgNPs3 conjugate. Gram+ and Gram- bacterial isolates, i.e., , and , were used to test the antibacterial activities of newly developed CHI‒AgNPs conjugates. In comparison to the control sample with bacterial cell count 1.5 × 10⁸ CFU/mL, a notable reduction in the log values was recorded for the CHI‒AgNPs3 conjugate. The antibiofilm potential of CHI‒AgNPs conjugates was tested against . Moreover, the CHI‒AgNPs3 conjugate also showed substantial cytotoxicity against the MCF-7 (breast cancer) cell line. In summary, the newly engineered CHI‒AgNPs conjugates with antibacterial, antibiofilm, and anticancer potentialities are potential candidate materials for biomedical applications.
在 21 世纪，随着人类意识和社会意识的不断提高，研究人员必须应对那些对人类安全构成重大威胁的微生物感染。由于多种原因，威胁病原体的出现/再现有所增加，对医疗保健服务构成了严重挑战。考虑到具有医疗潜力的 21 世纪材料的动态变化，将生物活性物质整合到材料中以构建抗菌基质的研究还很有限。因此，抗菌活性缀合物被认为是消除感染和减少医疗机构中微生物污染的潜在候选物。在这种情况下，使用从 () 提取物和壳聚糖 (CHI) 中生物合成的银纳米粒子 (AgNPs) 开发了具有抗菌、抗生物膜和抗癌潜力的环保型新型缀合物。一系列仪器和成像工具，即紫外-可见和傅里叶变换红外光谱、扫描电子显微镜 (SEM)、透射电子显微镜 (TEM)、能谱 (EDX) 和 X 射线衍射 (XRD)，用于表征新提取的 AgNPs。在 24 小时反应期后获得的生物合成 AgNPs 用于构建基于 CHI 的缀合物，并根据 AgNPs 浓度指定为 CHI-AgNPs1 至 CHI-AgNPs5。在规定的加载周期后，CHI-AgNPs3 缀合物记录到 92%的加载效率 (LE)。革兰氏阳性和革兰氏阴性细菌分离株，即 、 和 ，用于测试新开发的 CHI-AgNPs 缀合物的抗菌活性。与细菌细胞计数为 1.5×108 CFU/mL 的对照样品相比，CHI-AgNPs3 缀合物的对数显著降低。CHI-AgNPs 缀合物的抗生物膜潜力针对 进行了测试。此外，CHI-AgNPs3 缀合物对 MCF-7(乳腺癌)细胞系也表现出显著的细胞毒性。总之，具有抗菌、抗生物膜和抗癌潜力的新型工程 CHI-AgNPs 缀合物是生物医学应用的潜在候选材料。
