Department of Two Branches of Hepatobiliary Surgery, Yantai Yuhuangding Hospital, Yantai, China.
Eur Rev Med Pharmacol Sci. 2020 Sep;24(18):9325-9332. doi: 10.26355/eurrev_202009_23014.
The purpose of this paper was to explore roles and significance of transcriptional repressor GATA binding 1 (TRPS1) in the process of hepatocellular carcinoma (HCC) and clinicopathological parameters of HCC patients, respectively.
The expression of TRPS1 was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and Western blot. Chi-square test was used to investigate the association between TRPS1 and clinicopathological parameters of HCC patients. SiRNA was designed to suppress the expression of TRPS1. Moreover, transwell assay and cell cycle analysis were adopted to determine the invasion ability and proliferation ability of HCC cells.
It was demonstrated that TRPS1 was abnormally over-expressed in HCC tissues and cells, and was closely associated with the vascular invasion, tumor size, and TNM stage of HCC patients. Besides, the results indicated that the inhibition of TRPS1 in HCC cells could impede the cell invasion ability and proliferation ability.
TRPS1 plays a key role in the development of HCC and is closely associated with the clinicopathological parameters of HCC patients. Hence, it is proposed that TRPS1 may serve as a novel prognostic marker and a potential therapeutic target for HCC treatment.
本文旨在分别探讨转录抑制因子 GATA 结合蛋白 1（TRPS1）在肝细胞癌（HCC）发生发展过程中的作用及其与 HCC 患者临床病理参数的关系。
采用实时荧光定量聚合酶链式反应（qRT-PCR）和 Western blot 检测 TRPS1 的表达情况。采用卡方检验分析 TRPS1 与 HCC 患者临床病理参数的关系。设计 siRNA 以抑制 TRPS1 的表达。采用 Transwell 实验和细胞周期分析检测 HCC 细胞的侵袭能力和增殖能力。
结果表明，TRPS1 在 HCC 组织和细胞中异常高表达，与 HCC 患者的血管侵犯、肿瘤大小和 TNM 分期密切相关。此外，研究结果表明，抑制 HCC 细胞中的 TRPS1 可抑制细胞的侵袭能力和增殖能力。
TRPS1 在 HCC 的发生发展中起关键作用，并与 HCC 患者的临床病理参数密切相关。因此，TRPS1 可能作为 HCC 治疗的一种新的预后标志物和潜在的治疗靶点。
