Department of Gastroenterology, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, China.
Eur Rev Med Pharmacol Sci. 2020 Sep;24(18):9400-9407. doi: 10.26355/eurrev_202009_23023.
The aim of this study was to explore the expression of long non-coding ribonucleic acid HEIH (lncRNA-HEIH) in gastric cancer (GC) tissues, and to investigate its effects on the proliferation, apoptosis and invasion of HGC-27 cells.
A total of 80 tissue samples were collected from patients diagnosed with GC in Shenzhen People's Hospital. Meanwhile, para-carcinoma tissues were enrolled as normal controls (Control group). Total RNA was extracted from tissues, and the expression of lncRNA-HEIH was detected via quantitative reverse transcription-polymerase chain reaction (qRT-PCR). HGC-27 cells were cultured and transfected with small-interfering RNA-HEIH (si-HEIH group). At 48 h after transfection, cell proliferation, apoptosis and invasion were detected via methyl thiazolyl tetrazolium (MTT) assay, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay and transwell assay, respectively.
(1) Compared with Control group, the expression of lncRNA-HEIH rose significantly in GC tissues (p<0.01). (2) The expression of lncRNA-HEIH in HGC-27 cells was significantly down-regulated in si-HEIH group compared with si-NC group (p<0.01). (3) Compared with those in si-NC group, the proliferation of HGC-27 cells was suppressed (p<0.05), while the apoptosis of HGC-27 cells was promoted (p<0.01) in si-HEIH group. (4) The invasion of HGC-27 cells was remarkably inhibited in Si-HEIH group than si-NC group (p<0.05).
LncRNA-HEIH is highly expressed in GC patients, which affects the proliferation, apoptosis and invasion of GC HGC-27 cells.
本研究旨在探讨长链非编码核糖核酸 HEIH（lncRNA-HEIH）在胃癌（GC）组织中的表达，并探讨其对 HGC-27 细胞增殖、凋亡和侵袭的影响。
收集深圳市人民医院 80 例 GC 患者组织标本，同时选取癌旁组织作为正常对照（对照组）。提取组织总 RNA，采用实时定量逆转录聚合酶链反应（qRT-PCR）检测 lncRNA-HEIH 的表达。HGC-27 细胞培养后转染小干扰 RNA-HEIH（si-HEIH 组）。转染 48 h 后，采用噻唑蓝（MTT）比色法、末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记法（TUNEL）和 Transwell 实验分别检测细胞增殖、凋亡和侵袭情况。
（1）与对照组相比，GC 组织中 lncRNA-HEIH 的表达明显升高（p<0.01）。（2）与 si-NC 组相比，si-HEIH 组 HGC-27 细胞中 lncRNA-HEIH 的表达明显下调（p<0.01）。（3）与 si-NC 组相比，si-HEIH 组 HGC-27 细胞的增殖受到抑制（p<0.05），凋亡受到促进（p<0.01）。（4）与 si-NC 组相比，si-HEIH 组 HGC-27 细胞的侵袭明显受到抑制（p<0.05）。
lncRNA-HEIH 在 GC 患者中高表达，影响 GC HGC-27 细胞的增殖、凋亡和侵袭。
