Department of Orthopedics, Taizhou People's Hospital, 366 Taihu Road, Taizhou, Jiangsu, China.
Department of Oncology, The Affiliated Suzhou Hospital of Nanjing Medical University, 242 Guangji Road, Suzhou 215008, Jiangsu, China.
J Biochem. 2021 Jul 3;169(5):535-542. doi: 10.1093/jb/mvaa134.
The present study aimed to investigate the function of long non-coding RNA HEIH in gastric carcinoma (GC). Adjacent normal tissues and GC tissues were obtained from 72 patients. Real-time quantitative polymerase chain reaction (RT-qPCR) was utilized to measure the expression of HEIH in cancer tissues and cells. Cell Counting Kit-8 and transwell assays were employed to evaluate cell proliferation, migration and invasion. An Annexin V-fluorescein-isothiocyanate (FITC)/propidium iodide (PI) Apoptosis Detection Kit was used to evaluate the apoptosis ratio. RT-qPCR was used to detect the expression level of miR-214-3p. The expression of HEIH in GC tissues was higher than in adjacent normal tissues. The expression of HEIH was upregulated in MKN-45, NCL-N87, KATO III cell lines compared within normal gastric epithelial cells. Knockdown of lncRNA HEIH significantly decreased the number of migrated and invaded cells. Additionally, downregulation of HEIH could increase GC cell apoptosis compared with the non-specific control (NC) group. We also proved that miR-214-3p was the direct target of lncRNA HEIH, and that overexpression of miR-214-3p could reverse the effects of HEIH. Silencing of HEIH could suppress Gastric Carcinoma cell proliferation, migration and invasion by inhibiting miR-214-3p. Thus, HEIH might represent a novel biomarker and therapeutic target.
本研究旨在探讨长链非编码 RNA HEIH 在胃癌（GC）中的作用。从 72 名患者中获得了相邻正常组织和 GC 组织。实时定量聚合酶链反应（RT-qPCR）用于测量癌症组织和细胞中 HEIH 的表达。细胞计数试剂盒-8 和 Transwell 分析用于评估细胞增殖、迁移和侵袭。Annexin V-荧光素异硫氰酸酯（FITC）/碘化丙啶（PI）凋亡检测试剂盒用于评估凋亡率。RT-qPCR 用于检测 miR-214-3p 的表达水平。GC 组织中 HEIH 的表达高于相邻正常组织。与正常胃上皮细胞相比，MKN-45、NCL-N87、KATO III 细胞系中 HEIH 的表达上调。lncRNA HEIH 的敲低显着减少了迁移和侵袭细胞的数量。此外，与非特异性对照（NC）组相比，下调 HEIH 可增加 GC 细胞凋亡。我们还证明了 miR-214-3p 是 lncRNA HEIH 的直接靶标，而过表达 miR-214-3p 可以逆转 HEIH 的作用。沉默 HEIH 可以通过抑制 miR-214-3p 来抑制胃癌细胞的增殖、迁移和侵袭。因此，HEIH 可能代表一种新的生物标志物和治疗靶点。
