长链非编码 RNA SNHG16 通过调节 miR-520/VEGF 轴促进肺癌细胞的增殖、迁移和侵袭。
LncRNA SNHG16 drives proliferation, migration, and invasion of lung cancer cell through modulation of miR-520/VEGF axis.
机构信息
Department of Cardio-Thoracic Surgery, Zhangqiu District People's Hospital, Jinan, P.R. China.
出版信息
Eur Rev Med Pharmacol Sci. 2020 Sep;24(18):9522-9531. doi: 10.26355/eurrev_202009_23037.
OBJECTIVE
Long non-coding RNAs (lncRNAs) have been shown to have important effects on various biological behavior of human diseases. Although increasing lncRNAs have been explored in human cancers, there are still countless lncRNA to be mined. The purpose of this study was to investigate the effect of lncRNA SNHG16 on the proliferation and metastasis of lung cancer cells.
PATIENTS AND METHODS
RT-qPCR was used to analyze the expression patterns of SNHG16, miR-520 and VEGF. MTT and transwell methods were used to detect the effect of SNHG16 on cell migration. The association between SNHG16, miR-520 and VEGF was analyzed by bioinformatics analysis and Dual-Luciferase verification reporter analysis. Finally, lung cancer cells have demonstrated the role of SNHG16-miR-520-VEGF in the cell biological behavior axis.
RESULTS
Compared with normal cells, SNHG16 is highly expressed in lung cancer cells. Silent SNHG16 has a negative effect on the migration of lung cancer cells.
CONCLUSIONS
LncRNA SNHG16 as ceRNA up-regulates VEGF in lung cancer cells by binding to miR-520. LncRNA SNHG16 as ceRNA promotes the migration of lung cancer cells by regulating the miR-520/VEGF axis.
目的
长链非编码 RNA（lncRNAs）已被证明对人类疾病的各种生物学行为具有重要影响。尽管已经在人类癌症中探索了越来越多的 lncRNA，但仍有无数的 lncRNA有待挖掘。本研究旨在探讨 lncRNA SNHG16 对肺癌细胞增殖和转移的影响。
患者和方法
使用 RT-qPCR 分析 SNHG16、miR-520 和 VEGF 的表达模式。MTT 和 Transwell 方法用于检测 SNHG16 对细胞迁移的影响。通过生物信息学分析和 Dual-Luciferase 验证报告分析来分析 SNHG16、miR-520 和 VEGF 之间的关联。最后，肺癌细胞证实了 SNHG16-miR-520-VEGF 在细胞生物学行为轴中的作用。
结果
与正常细胞相比，SNHG16 在肺癌细胞中高表达。沉默 SNHG16 对肺癌细胞的迁移具有负向影响。
结论
lncRNA SNHG16 作为 ceRNA 通过与 miR-520 结合上调肺癌细胞中的 VEGF。lncRNA SNHG16 作为 ceRNA 通过调节 miR-520/VEGF 轴促进肺癌细胞的迁移。
Zotero 插件