利福平对RNA噬菌体f2感染性的影响。

Effect of rifampicin on the infectivity of RNA bacteriophage f2.

作者信息

Naimski P, Chroboczek J

出版信息

Eur J Biochem. 1977 Jun 15;76(2):419-23. doi: 10.1111/j.1432-1033.1977.tb11610.x.

DOI:10.1111/j.1432-1033.1977.tb11610.x

Abstract

RNA bacteriophage f2, treated in vitro with rifampicin, loses infectivity dramatically. Rifampicin interacts with phage RNA, binding to a few specific sites. Inhibition of phage RNA infectivity occurs at 10-100 times lower molar excess of rifampicin than inhibition of infectivity of intact phage particles. Thus the phage capsid acts as a barrier, diminishing interaction of the drug with phage RNA.

摘要

用利福平在体外处理RNA噬菌体f2，其感染力会显著丧失。利福平与噬菌体RNA相互作用，结合到一些特定位点。抑制噬菌体RNA感染力所需的利福平摩尔过量比抑制完整噬菌体颗粒感染力时低10至100倍。因此，噬菌体衣壳起到了屏障作用，减少了药物与噬菌体RNA的相互作用。