噬菌体N4发育所需的病毒体相关RNA聚合酶。
Virion-associated RNA polymerase required for bacteriophage N4 development.
作者信息
Falco S C, Laan K V, Rothman-Denes L B
出版信息
Proc Natl Acad Sci U S A. 1977 Feb;74(2):520-3. doi: 10.1073/pnas.74.2.520.
Abstract
Escherichia coli phage N4 transcription is resistant to rifampicin, an inhibitor of the host RNA polymerase, even when the drug is added prior to infection. A rifampicin-resistant RNA polymerase has been detected in disrupted N4 virions. This enzyme shows a requirement for the four ribonucleoside 5'-triphosphates and exogenous denatured DNA. With denatured N4 DNA, the preferred template, transcription is asymmetric. The virion RNA polymerase apparently is necessary for phage development because a conditional lethal N4 mutant shows temperature-sensitive RNA synthesis in vivo as well as a temperature-sensitive RNA polymerase in disrupted virions.
摘要
大肠杆菌噬菌体N4的转录对利福平（一种宿主RNA聚合酶抑制剂）具有抗性，即使在感染前加入该药物也是如此。在破碎的N4病毒粒子中检测到一种抗利福平的RNA聚合酶。这种酶需要四种核糖核苷5'-三磷酸和外源变性DNA。以变性的N4 DNA（首选模板）进行转录时是不对称的。病毒粒子RNA聚合酶显然是噬菌体发育所必需的，因为一个条件致死的N4突变体在体内表现出温度敏感的RNA合成，并且在破碎的病毒粒子中也有温度敏感的RNA聚合酶。
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