Jeon Gibeom, Ahn Juhee
Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
FEMS Microbiol Lett. 2020 Oct 16;367(19). doi: 10.1093/femsle/fnaa159.
This study was designed to evaluate the synergistic effect of phage (P22) and antibiotic on the inhibition of Salmonella Typhimurium exposed to ceftriaxone (CEF) and ciprofloxacin (CIP). The effect of phage and antibiotic treatments was evaluated by plaque size, disk diffusion, antibiotic susceptibility and phage multiplication assays. The sequential treatment effect of phage and antibiotic was carried out in different treatment order and time for 12 h at 37°C. P22 plaque sizes were increased by 28 and 71%, respectively, in the presence of CEF and CIP. The clear zone sizes in disk diffusion assay were significantly increased to >37 mm in the presence of CEF and CIP compared to the control (28-31 mm). Pre-treatment with P22 enhanced the antimicrobial effect of CIP, showing >2 log reduction after a 12 h incubation. Phage P22 combined with antibiotics (CEF and CIP) effectively inhibited the growth of S. Typhimurium depending on the treatment order and time. These results provide useful information for understanding the synergistic effect of phage and antibiotic treatment which can be an effective option to control antibiotic resistant pathogens.
本研究旨在评估噬菌体（P22）与抗生素联合使用对抑制暴露于头孢曲松（CEF）和环丙沙星（CIP）的鼠伤寒沙门氏菌的协同作用。通过噬菌斑大小、纸片扩散法、抗生素敏感性和噬菌体增殖试验评估噬菌体和抗生素处理的效果。噬菌体和抗生素的序贯处理效果在不同的处理顺序和时间下于37°C进行12小时。在存在CEF和CIP的情况下，P22噬菌斑大小分别增加了28%和71%。与对照（28 - 31毫米）相比，在存在CEF和CIP的情况下，纸片扩散试验中的透明圈大小显著增加至>37毫米。用P22预处理增强了CIP的抗菌效果，孵育12小时后显示出>2个对数级的降低。噬菌体P22与抗生素（CEF和CIP）联合使用根据处理顺序和时间有效地抑制了鼠伤寒沙门氏菌的生长。这些结果为理解噬菌体和抗生素联合治疗的协同作用提供了有用信息，这可能是控制抗生素耐药病原体的有效选择。
