Ostrand-Rosenberg S, Clements V
Immunogenetics. 1987;26(1-2):1-5. doi: 10.1007/BF00345447.
The 402AX teratocarcinoma is a 129/J-derived mouse major histocompatibility complex (MHC) antigen negative tumor that is induced to express H-2b class I antigens during rejection. Resistance to 402AX by MHC allogeneic and syngeneic mice is immunologically mediated and involves the recognition of tumor-associated antigens (TAA) in the context of induced MHC class I antigens. The current studies were undertaken to define the 402AX TAAs. Reconstitution of irradiated susceptible hosts (129/J) with 402AX-primed resistant spleen cells (C57BL/6) results in acute graft-versus-host disease, suggesting that tumor-primed C57BL/6 splenocytes are reactive to tumor genotype (129/J) minor histocompatibility (Hm) antigens. C57BL/6 anti-129/J effector cells, although not directly cytotoxic for 402AX cells, are specifically cold target inhibited by 402AX cells. Genetically susceptible hosts (C3H.SW) immunized to 129/J Hm antigens by skin grafting become resistant to an i.p. challenge of 402AX cells. These results suggest that 129/J Hm antigens may be the TAAs recognized during genetically controlled rejection of the 402AX teratocarcinoma.
402AX畸胎癌是一种源自129/J小鼠的主要组织相容性复合体（MHC）抗原阴性肿瘤，在排斥反应过程中可诱导其表达H-2b I类抗原。MHC同种异体和同基因小鼠对402AX的抗性是由免疫介导的，并且涉及在诱导的MHC I类抗原背景下对肿瘤相关抗原（TAA）的识别。当前的研究旨在确定402AX TAA。用经402AX致敏的抗性脾细胞（C57BL/6）重建受辐照的易感宿主（129/J）会导致急性移植物抗宿主病，这表明经肿瘤致敏的C57BL/6脾细胞对肿瘤基因型（129/J）的次要组织相容性（Hm）抗原具有反应性。C57BL/6抗129/J效应细胞虽然对402AX细胞没有直接细胞毒性，但会被402AX细胞特异性地抑制冷靶细胞。通过皮肤移植免疫129/J Hm抗原的遗传易感宿主（C3H.SW）对402AX细胞的腹腔注射攻击产生抗性。这些结果表明，129/J Hm抗原可能是在对402AX畸胎癌进行基因控制的排斥反应过程中被识别的TAA。
