Dep. of Plant Sciences, Crop Development Centre, Univ. of Saskatchewan, Saskatoon, Saskatchewan, S7N 5A8, Canada.
ICRISAT, Hyderabad, 502324, India.
Plant Genome. 2019 Nov;12(3):1-12. doi: 10.3835/plantgenome2019.07.0067.
Quantitative trait locus (QTL) analyses for carotenoids in chickpea were completed for three F populations. A moderate number of QTLs and candidate genes associated with carotenoid concentration in chickpea seeds were identified. Green cotyledon color is positively associated with provitamin A carotenoids. Three F populations derived from crosses between cultivars with green and yellow cotyledon colors were used to identify quantitative trait loci (QTLs) associated with carotenoid components in chickpea (Cicer arietinum L.) seeds developed by the Crop Development Centre (CDC). Carotenoids including violaxanthin, lutein, zeaxanthin, β-cryptoxanthin, and β-carotene were assessed in the F seeds via high-performance liquid chromatography (HPLC). In the 'CDC Jade' × 'CDC Frontier' population, 1068 bin markers derived from the 50K Axiom CicerSNP array were mapped onto eight linkage groups (LGs). Eight QTLs, including two each for β-carotene and zeaxanthin and one each for total carotenoids, β-cryptoxanthin, β-carotene, and violaxanthin were identified in this population. In the 'CDC Cory' × 'CDC Jade' population, 694 bin markers were mapped onto eight LGs and one partial LG. Quantitative trait loci for β-cryptoxanthin, β-carotene, violaxanthin, lutein, and total carotenoids were identified on LG8. A map with eight LGs was developed from 581 bin markers in the third population derived from the 'ICC4475' × 'CDC Jade' cross. One QTL for β-carotene and four QTLs, one each for β-cryptoxanthin, β-carotene, lutein, and total carotenoids, were identified in this population. The highest phenotypic variation explained by the QTLs was for β-carotene, which ranged from 58 to 70% in all three populations. A major gene for cotyledon color was mapped on LG8 in each population. A significant positive correlation between cotyledon color and carotenoid concentration was observed. Potential candidate genes associated with carotenoid components were obtained and their locations on the chickpea genome are presented.
针对三个 F 群体，完成了对鹰嘴豆类胡萝卜素的数量性状位点（QTL）分析。鉴定出与鹰嘴豆种子类胡萝卜素浓度相关的大量 QTL 和候选基因。绿色子叶颜色与维生素 A 前体类胡萝卜素呈正相关。使用源自绿色和黄色子叶品种杂交的三个 F 群体，鉴定与作物发展中心（CDC）开发的鹰嘴豆种子中类胡萝卜素成分相关的数量性状位点（QTL）。通过高效液相色谱法（HPLC）评估 F 种子中的类胡萝卜素，包括紫黄质、叶黄素、玉米黄质、β-隐黄质和β-胡萝卜素。在 'CDC Jade' × 'CDC Frontier' 群体中，来自 50K Axiom CicerSNP 数组的 1068 个 bin 标记映射到八个连锁群（LG）上。在该群体中鉴定出 10 个 QTL，包括β-胡萝卜素和玉米黄质各 2 个，总类胡萝卜素、β-隐黄质、β-胡萝卜素和紫黄质各 1 个。在 'CDC Cory' × 'CDC Jade' 群体中，694 个 bin 标记映射到八个 LG 和一个部分 LG。在 LG8 上鉴定出β-隐黄质、β-胡萝卜素、紫黄质、叶黄素和总类胡萝卜素的数量性状位点。从 'ICC4475' × 'CDC Jade' 杂交衍生的第三个群体中，581 个 bin 标记开发了一个具有八个 LG 的图谱。在该群体中鉴定出 1 个β-胡萝卜素 QTL 和 4 个 QTL，分别为β-隐黄质、β-胡萝卜素、叶黄素和总类胡萝卜素各 1 个。在所有三个群体中，由 QTL 解释的最高表型变异为β-胡萝卜素，范围为 58%至 70%。在每个群体中，子叶颜色的主基因都映射到 LG8 上。观察到子叶颜色与类胡萝卜素浓度之间存在显著正相关。获得了与类胡萝卜素成分相关的潜在候选基因，并展示了它们在鹰嘴豆基因组上的位置。
