Abbo S, Molina C, Jungmann R, Grusak M A, Berkovitch Z, Reifen Ruth, Kahl G, Winter P, Reifen R
Institute of Plant Science and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel.
Theor Appl Genet. 2005 Jul;111(2):185-95. doi: 10.1007/s00122-005-1930-y. Epub 2005 May 26.
Chickpea is a staple protein source in many Asian and Middle Eastern countries. The seeds contain carotenoids such as beta-carotene, cryptoxanthin, lutein and zeaxanthin in amounts above the engineered beta-carotene-containing "golden rice" level. Thus, breeding for high carotenoid concentration in seeds is of nutritional, socio-economic, and economic importance. To study the genetics governing seed carotenoids in chickpea, we studied the relationship between seed weight and concentrations of beta-carotene and lutein by means of high-performance liquid chromatography in segregating progeny from a cross between an Israeli cultivar and wild Cicer reticulatum Ladiz. Seeds of the cross progeny varied with respect to their carotenoid concentration (heritability estimates ranged from 0.5 to 0.9), and a negative genetic correlation was found between mean seed weight and carotenoid concentration in the F(3). To determine the loci responsible for the genetic variation observed, the population was genotyped using 91 sequence tagged microsatellite site markers and two CytP450 markers to generate a genetic map consisting of nine linkage groups and a total length of 344.6 cM. Using quantitative data collected for beta-carotene and lutein concentration and seed weight of the seeds of the F(2) population, we were able to identify quantitative trait loci (QTLs) by interval mapping. At a LOD score of 2, four QTLs for beta-carotene concentration, a single QTL for lutein concentration and three QTLs for seed weight were detected. The results of this investigation may assist in improving the nutritional quality of chickpea.
鹰嘴豆是许多亚洲和中东国家的主要蛋白质来源。其种子中含有的类胡萝卜素，如β-胡萝卜素、隐黄质、叶黄素和玉米黄质，含量高于经过基因改造的含β-胡萝卜素的“黄金大米”。因此，培育种子中类胡萝卜素浓度高的品种具有营养、社会经济和经济方面的重要意义。为了研究鹰嘴豆种子类胡萝卜素的遗传规律，我们通过高效液相色谱法研究了以色列品种与野生鹰嘴豆（Cicer reticulatum Ladiz）杂交后代中种子重量与β-胡萝卜素和叶黄素浓度之间的关系。杂交后代的种子类胡萝卜素浓度各不相同（遗传力估计值在0.5至0.9之间），并且在F(3)代中发现平均种子重量与类胡萝卜素浓度之间存在负遗传相关性。为了确定导致观察到的遗传变异的基因座，使用91个序列标签微卫星位点标记和两个细胞色素P450标记对该群体进行基因分型，以生成一个由9个连锁群组成、总长度为344.6 cM的遗传图谱。利用收集到的F(2)群体种子的β-胡萝卜素和叶黄素浓度以及种子重量的定量数据，我们能够通过区间作图法鉴定数量性状基因座（QTL）。在LOD值为2时，检测到4个β-胡萝卜素浓度的QTL、1个叶黄素浓度的QTL和3个种子重量的QTL。本研究结果可能有助于改善鹰嘴豆的营养品质。
