Mato Grosso Do Sul State University (UEMS), Natural Resources Study Center (CERNA), Laboratory of Ecology, Brazil.
Mato Grosso Do Sul State University (UEMS), Natural Resources Study Center (CERNA), Laboratory of Ecology, Brazil; Postgraduate Program in Natural Resources, Mato Grosso Do Sul State University, Rod. Dourados Itahum Km 12, Dourados, MS, 79804-970, Brazil.
Chemosphere. 2021 Feb;264(Pt 1):128383. doi: 10.1016/j.chemosphere.2020.128383. Epub 2020 Sep 21.
This study aimed to evaluate water quality using limnological parameters in two sub-basins of the Negro and Apa Rivers, along with the composition and structure of the landscape around the locations sampled in the two sub-basins and their possible contribution to the loss of water quality. We also set out to identify the genotoxic effects on the erythrocytes of Prochilodus lineatus and Leporinus friderici. In particular, we quantified total Cr, Ni and Pb in the muscle of these fish species to determine which genotoxic changes are related to the concentration of metals detected in the fish from these two sub-basins of the Paraguay River Basin. The sub-basin of the Apa River presented a greater proportion of surrounding pastureland in relation to the basin of the Negro River. The erythrocytes of P. lineatus showed a significant difference between the sub-basins for nuclear pyknosis, binucleated cells and lobed nuclei (p < 0.05). In L. friderici, vacuolated nuclei, nuclear pyknosis and lobed nuclei alterations also showed a significant difference between the sub-basins (p < 0.05). The genotoxicity index of the Apa River sub-basin presented higher frequencies of nuclear alterations in P. lineatus and L. friderici (p < 0.05), compared to the Negro River sub-basin. The concentration of total Cr, Ni and Pb in the muscles of P. lineatus and L. friderici in the Negro River sub-basin leads to genotoxic damage, mainly from the induction of lobed nuclei. Based on our results, it can be concluded that toxicogenetic effects in native fish species are an important source of environmental diagnosis in the Upper Paraguay River Basin.
本研究旨在通过内比都和阿帕河流域的两个子流域的湖泊学参数来评估水质，以及采样地点周围景观的组成和结构及其对水质下降的可能贡献。我们还旨在确定 Prochilodus lineatus 和 Leporinus friderici 红细胞的遗传毒性作用。特别是，我们量化了这些鱼类肌肉中的总 Cr、Ni 和 Pb，以确定与这两个巴拉圭河流域子流域鱼类中检测到的金属浓度相关的遗传毒性变化。阿帕河流域与内比都河流域相比，周围的牧场比例更大。P. lineatus 红细胞在子流域之间的核固缩、双核细胞和分叶核方面存在显著差异（p < 0.05）。在 L. friderici 中，空泡核、核固缩和分叶核改变也在子流域之间存在显著差异（p < 0.05）。与内比都河流域相比，阿帕河流域的遗传毒性指数在 P. lineatus 和 L. friderici 中表现出更高的核改变频率（p < 0.05）。P. lineatus 和 L. friderici 肌肉中的总 Cr、Ni 和 Pb 浓度导致遗传毒性损伤，主要来自诱导分叶核。根据我们的结果，可以得出结论，有毒遗传效应在本地鱼类中是上巴拉圭河流域环境诊断的重要来源。
