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天蓝色链霉菌A3(2)假定蛋白（SCO0618）的分子特征及功能注释

Molecular characterization and functional annotation of a hypothetical protein (SCO0618) of Streptomyces coelicolor A3(2).

作者信息

Ferdous Nadim, Reza Mahjerin Nasrin, Emon Md Tabassum Hossain, Islam Md Shariful, Mohiuddin A K M, Hossain Mohammad Uzzal

机构信息

Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Tangail 1902, Bangladesh.

Laboratory of Reproductive and Developmental Biology, Hokkaido University, Sapporo 060-0808, Japan.

出版信息

Genomics Inform. 2020 Sep;18(3):e28. doi: 10.5808/GI.2020.18.3.e28. Epub 2020 Sep 21.

DOI:10.5808/GI.2020.18.3.e28
PMID:33017872
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7560446/
Abstract

Streptomyces coelicolor is a gram-positive soil bacterium which is well known for the production of several antibiotics used in various biotechnological applications. But numerous proteins from its genome are considered hypothetical proteins. Therefore, the present study aimed to reveal the functions of a hypothetical protein from the genome of S. coelicolor. Several bioinformatics tools were employed to predict the structure and function of this protein. Sequence similarity was searched through the available bioinformatics databases to find out the homologous protein. The secondary and tertiary structure were predicted and further validated with quality assessment tools. Furthermore, the active site and the interacting proteins were also explored with the utilization of CASTp and STRING server. The hypothetical protein showed the important biological activity having with two functional domain including POD-like_MBL-fold and rhodanese homology domain. The functional annotation exposed that the selected hypothetical protein could show the hydrolase activity. Furthermore, protein-protein interactions of selected hypothetical protein revealed several functional partners those have the significant role for the bacterial survival. At last, the current study depicts that the annotated hypothetical protein is linked with hydrolase activity which might be of great interest to the further research in bacterial genetics.

摘要

天蓝色链霉菌是一种革兰氏阳性土壤细菌，以产生多种用于各种生物技术应用的抗生素而闻名。但其基因组中的许多蛋白质被认为是假定蛋白质。因此，本研究旨在揭示天蓝色链霉菌基因组中一种假定蛋白质的功能。使用了几种生物信息学工具来预测该蛋白质的结构和功能。通过可用的生物信息学数据库搜索序列相似性，以找出同源蛋白质。预测了二级和三级结构，并用质量评估工具进一步验证。此外，还利用CASTp和STRING服务器探索了活性位点和相互作用蛋白。该假定蛋白质显示出重要的生物学活性，具有包括POD样_MBL折叠和硫氧还蛋白同源结构域在内的两个功能结构域。功能注释表明，所选的假定蛋白质可能具有水解酶活性。此外，所选假定蛋白质的蛋白质-蛋白质相互作用揭示了几个对细菌生存具有重要作用的功能伙伴。最后，本研究表明，注释的假定蛋白质与水解酶活性有关，这可能对细菌遗传学的进一步研究具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4073/7560446/510ab6c5b027/gi-2020-18-3-e28f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4073/7560446/8c052fe53a06/gi-2020-18-3-e28f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4073/7560446/7bbf9844e6b4/gi-2020-18-3-e28f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4073/7560446/3678b91509da/gi-2020-18-3-e28f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4073/7560446/014a19329f5d/gi-2020-18-3-e28f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4073/7560446/abc39768b480/gi-2020-18-3-e28f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4073/7560446/0aeff6e05a6c/gi-2020-18-3-e28f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4073/7560446/c19b504f8570/gi-2020-18-3-e28f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4073/7560446/510ab6c5b027/gi-2020-18-3-e28f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4073/7560446/8c052fe53a06/gi-2020-18-3-e28f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4073/7560446/7bbf9844e6b4/gi-2020-18-3-e28f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4073/7560446/3678b91509da/gi-2020-18-3-e28f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4073/7560446/014a19329f5d/gi-2020-18-3-e28f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4073/7560446/abc39768b480/gi-2020-18-3-e28f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4073/7560446/0aeff6e05a6c/gi-2020-18-3-e28f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4073/7560446/c19b504f8570/gi-2020-18-3-e28f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4073/7560446/510ab6c5b027/gi-2020-18-3-e28f8.jpg

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