Feshki Mohamad, Monfared Mohamad Sadegh, Gosselin Benoit
Annu Int Conf IEEE Eng Med Biol Soc. 2020 Jul;2020:1836-1839. doi: 10.1109/EMBC44109.2020.9176191.
Measuring neural activity from well-defined neural populations deep inside the brain has an important value in neuroscience. Fiber photometry is an important technique for evaluating neuron activity inside the brain. Besides, miniature wireless systems to record neuronal activity in a fully untethered experimental setting have recently become extremely interesting for experimenters. Still, a noise-robust wireless fiber photometry system for this purpose does not exist. Using an isosbestic excitation wavelength for recording with GCaMP6 has recently been suggested to reduce the different types of noises. We present the design of a wireless fiber photometry system at 470 nm for calcium-dependent fluorescence emission of GCaMP6 using a calcium-independent isosbestic excitation wavelength of 410 nm. Synthetic emission fluorescence light was played from a function generator to drive an LED at 530 nm at test the photometry platform. The setup has been fixed at 4.18 mW light power after linearity assessment while the analog circuit has THD of 0.35%. Then, the recorded synthetic neuronal activity was transmitted wirelessly to the base station. Finally, the isosbestic response has been aligned and removed from the calcium-dependent fluorescence signal to have a noiseless neuronal activity.
测量大脑深处明确的神经群体的神经活动在神经科学中具有重要价值。光纤光度法是评估大脑内部神经元活动的一项重要技术。此外，在完全不受束缚的实验环境中记录神经元活动的微型无线系统最近引起了实验者的极大兴趣。然而，目前还不存在用于此目的的抗噪声无线光纤光度系统。最近有人建议使用等吸收激发波长来记录GCaMP6，以减少不同类型的噪声。我们展示了一种无线光纤光度系统的设计，该系统在470 nm处用于GCaMP6的钙依赖性荧光发射，使用410 nm的钙独立等吸收激发波长。从函数发生器播放合成发射荧光来驱动530 nm的发光二极管以测试光度平台。在线性评估后，该装置的光功率固定为4.18 mW，而模拟电路的总谐波失真为0.35%。然后，记录的合成神经元活动被无线传输到基站。最后，从钙依赖性荧光信号中对齐并去除等吸收响应，以获得无噪声的神经元活动。
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