Tamboli Suhel, Topolnik Dimitry, Radhakrishnan Risna, Veilleux-Lemieux Daphnée, Topolnik Lisa
Neuroscience Axis, CRCHUQ-CHUL, Quebec City, PQ, Canada; Department of Biochemistry, Microbiology, and Bioinformatics, Université Laval, Quebec City, PQ, Canada.
Direction des services vétérinaires, Université Laval, Quebec City, PQ, Canada.
STAR Protoc. 2024 Dec 20;5(4):103407. doi: 10.1016/j.xpro.2024.103407. Epub 2024 Oct 18.
Fiber photometry technique allows investigation of in vivo neural activity during behavior allowing understanding of brain-behavior relationship. Here, we provide a protocol for synchronized wireless fiber photometry and video recordings in rodents during behavior. We explain the detailed steps for stereotaxic virus injection, optic fiber cannula implantation, setup for synchronized fiber photometry and behavioral recording, and analysis of photometry data. These protocol steps can be adapted for various animal models, photometry, and behavioral recording systems. For complete details on the use and execution of this protocol, please refer to Tamboli et al. and Amalyan et al..
光纤光度测量技术能够在行为过程中对体内神经活动进行研究，从而有助于理解脑-行为关系。在此，我们提供了一种在啮齿动物行为过程中进行同步无线光纤光度测量和视频记录的方案。我们解释了立体定位病毒注射、光纤套管植入、同步光纤光度测量和行为记录设置以及光度测量数据分析的详细步骤。这些方案步骤可适用于各种动物模型、光度测量和行为记录系统。有关该方案使用和执行的完整详细信息，请参考Tamboli等人以及Amalyan等人的研究。
