Joseph G, Sharma C P
J Biomed Mater Res. 1987 Jul;21(7):937-45. doi: 10.1002/jbm.820210709.
The adhered platelets on artificial surfaces play a crucial role in inducing thrombosis. Therefore, it is very much desirable to have surfaces which can effectively retard platelet adhesion and aggregation. Prostaglandins like PGE2, PGE1 and PGD2 possess potent antiplatelet activity, but have very short half life. A chemically stable prostacyclin analog, 10,10-difluoro-13-dehydroprostacyclin (DF2-PGI2) seems to be promising. Polyelectrolyte (PE) synthesized from natural poly cis-1,4 isoprene has also been found to possess outstanding anticoagulant and antiplatelet activity. Modification of polyetherurethane urea (PEUU) by immobilizing DF2-PGI2 and poly-electrolyte in various combinations using glow discharge technique has been attempted. Surfaces thus modified showed negligible platelet adherence. The inhibition of platelet adherence in presence of inducing agents like fibrinogen, thrombin and ADP was also remarkable. The interactions of albumin and fibrinogen with the modified surfaces were studied using 125I labelled proteins.
人工表面上附着的血小板在引发血栓形成过程中起着关键作用。因此，非常需要有能够有效抑制血小板黏附和聚集的表面。诸如前列腺素E2、前列腺素E1和前列腺素D2等前列腺素具有强大的抗血小板活性，但半衰期很短。一种化学性质稳定的前列环素类似物，10,10-二氟-13-脱氢前列环素（DF2-PGI2）似乎很有前景。由天然聚顺式-1,4-异戊二烯合成的聚电解质（PE）也已被发现具有出色的抗凝血和抗血小板活性。人们尝试通过辉光放电技术以各种组合固定DF2-PGI2和聚电解质来对聚醚聚氨酯脲（PEUU）进行改性。如此改性后的表面显示出可忽略不计的血小板黏附。在存在纤维蛋白原、凝血酶和二磷酸腺苷等诱导剂的情况下对血小板黏附的抑制作用也很显著。使用125I标记的蛋白质研究了白蛋白和纤维蛋白原与改性表面的相互作用。
