Department of Advanced Functional Materials Science, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
Department of Biomaterials Science, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
Dent Mater. 2020 Dec;36(12):1586-1594. doi: 10.1016/j.dental.2020.09.009. Epub 2020 Oct 2.
Non-biodegradable particles comprising hydroxyethyl methacrylate (HEMA) and trimethylolpropane trimethacrylate (TMPT) have been reported as useful carriers for fibroblast growth factor-2 (FGF-2). They have also been successfully incorporated into the 4-[2-(methacryloyloxy)ethoxycarbonyl]phthalic anhydride/methyl methacrylate-tri-n-butyl borane (4-META/MMA-TBB) resin to promote tissue regeneration. However, smaller particles are required to obtain restorative materials acceptable for clinical use. The aim of this study was to investigate the ability of the 4-META/MMA-TBB resin that comprises small FGF-2-loaded particles to release FGF-2 and promote cell proliferation. In addition, the bonding and physical properties of the experimental resin were evaluated.
The small particles loaded with FGF-2 were newly fabricated and incorporated into the commercial 4-META/MMA-TBB resin. Release profiles of FGF-2 from the experimental resins were assessed, and the cell proliferation cultured with the eluate was evaluated. The bonding and physical properties of the resins were evaluated using shear bond strength and three-point bending tests, and by measuring the curing time, water absorption, and water dissolution.
Sustained release of FGF-2 from the experimental resins for two weeks was observed, and the released FGF-2 was demonstrated to promote cell proliferation. All bonding and physical properties of the 4-META/MMA-TBB resins were found acceptable for clinical use.
The small FGF-2-loaded particles incorporated into the 4-META/MMA-TBB resin had the same abilities to release FGF-2 and proliferate cells, as those exhibited by the conventionally sized particles. In addition, there were no adverse influences on bonding and physical properties, suggesting that the bioactive adhesive resin was acceptable for clinical use.
已报道包含羟乙基甲基丙烯酸酯（HEMA）和三羟甲基丙烷三甲基丙烯酸酯（TMPT）的不可生物降解颗粒可用作成纤维细胞生长因子-2（FGF-2）的有用载体。它们也已成功地掺入 4-[2-（甲基丙烯酰氧基）乙氧基羰基]邻苯二甲酸酐/甲基丙烯酸甲酯-三正丁基硼烷（4-META/MMA-TBB）树脂中以促进组织再生。然而，需要更小的颗粒来获得可接受的临床使用的修复材料。本研究的目的是研究包含载有较小 FGF-2 的颗粒的 4-META/MMA-TBB 树脂释放 FGF-2 和促进细胞增殖的能力。此外，还评估了实验树脂的粘结和物理性能。
新制备并掺入商业 4-META/MMA-TBB 树脂中的载有 FGF-2 的小颗粒。评估了实验树脂中 FGF-2 的释放曲线，并评估了用洗脱液培养的细胞增殖情况。使用剪切粘结强度和三点弯曲试验以及测量固化时间、吸水率和水溶解来评估树脂的粘结和物理性能。
观察到实验树脂中 FGF-2 的持续释放两周，并且释放的 FGF-2 被证明可促进细胞增殖。4-META/MMA-TBB 树脂的所有粘结和物理性能均被认为可用于临床。
掺入 4-META/MMA-TBB 树脂的小载有 FGF-2 的颗粒具有与常规大小的颗粒相同的释放 FGF-2 和增殖细胞的能力。此外，对粘结和物理性能没有不利影响，表明该生物活性粘结树脂可用于临床。
