Nasri Maryam, Adibhesami Glavizh, Mahdavifard Sina, Babaeenezhad Esmaeel, Ahmadvand Hassan
Razi Herbal Medicine Research Center, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Department of Biochemistry and Genetics, Lorestan University of Medical Sciences, Khorramabad, Iran.
Arch Physiol Biochem. 2023 Apr;129(2):363-372. doi: 10.1080/13813455.2020.1828478. Epub 2020 Oct 6.
This study aimed to evaluate the effects of glutamine (Gln) on diabetic nephropathy and other complications in a rat model of type 2 diabetes mellitus. Streptozotocin/nicotinamide induced diabetic rats were enrolled as an animal model of type 2 diabetes mellitus. Animals were divided into control, diabetic, and Gln (1000 mg/l in drinking water, eight weeks) treated diabetic groups. Gln alleviated renal inflammatory and oxidative stress biomarkers (tumour necrosis factor-alpha, interleukin 6, glutathione peroxidase, total superoxide dismutase, and glutathione), decreased serum uric acid and creatinine, and restored renal histopathological changes (glomerular volume, sclerosis, and leukocyte infiltration). Additionally, Gln ameliorated other complications, including systemic oxidative stress (serum malondialdehyde and nitric oxide, serum and liver glutathione, glutathione peroxidase, and total superoxide dismutase, and liver catalase), insulin resistance, hyperglycaemia, and hyperlipidaemia. Collectively, Gln attenuates diabetic nephropathy and other complications in type 2 diabetes mellitus in rats through its antioxidant and anti-inflammatory activities.
本研究旨在评估谷氨酰胺（Gln）对2型糖尿病大鼠模型中糖尿病肾病及其他并发症的影响。将链脲佐菌素/烟酰胺诱导的糖尿病大鼠作为2型糖尿病动物模型。动物被分为对照组、糖尿病组和Gln治疗糖尿病组（饮用水中含1000 mg/l，为期8周）。Gln减轻了肾脏炎症和氧化应激生物标志物（肿瘤坏死因子-α、白细胞介素6、谷胱甘肽过氧化物酶、总超氧化物歧化酶和谷胱甘肽），降低了血清尿酸和肌酐，并恢复了肾脏组织病理学变化（肾小球体积、硬化和白细胞浸润）。此外，Gln改善了其他并发症，包括全身氧化应激（血清丙二醛和一氧化氮、血清和肝脏谷胱甘肽、谷胱甘肽过氧化物酶、总超氧化物歧化酶和肝脏过氧化氢酶）、胰岛素抵抗、高血糖和高脂血症。总体而言，Gln通过其抗氧化和抗炎活性减轻了大鼠2型糖尿病中的糖尿病肾病及其他并发症。
