Mahdavifard Sina, Nakhjavani Manochehr
Department of Clinical Biochemistry, Faculty of Medical Sciences, Ardabil University of Medical Sciences, P.O. Box: 56189-85991, Ardabil, Iran.
Department of Endocrinology and Metabolism, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
J Diabetes Metab Disord. 2022 Jul 16;21(2):1233-1240. doi: 10.1007/s40200-021-00967-0. eCollection 2022 Dec.
The nuclear factor-kappa B (NF-κB) signaling participates in diabetes complications. Therefore, the reduction of NF-κB signaling may be a goal to prevent or improve them. Thus, we investigated the effects of acetylcysteine (AC), histidine (His), and their combination on the NF-κB expression and its different activators in type 2 diabetic rats.
The survey was performed on 50 rats that were allotted equally into five groups composed of control, diabetic, diabetic treated with (AC, 0.06%), (His, 0.1%), and (AC & His) groups. Treated groups have received the treatments daily in drinking water for two months. Metabolic profile (glucose, insulin resistance indices, lipid profile, and cardiovascular indices) and renal dysfunction parameters (creatinine and urinary protein excretion) were measured. Plus, diverse glycation (early, intermediate, and end), oxidative stress (Oxidized LDL, Reduced glutathione), and inflammatory markers (interleukine-1β, myeloperoxidase, and NF-kβ expression) were determined.
Glucose, insulin resistance indices, cardiovascular indices, renal dysfunction parameters, different markers of glycation, oxidative stress, and inflammation as well as NF-κB expression, were the lowest in the (AC & His) treated diabetic rats. Besides, the cited parameter was lower in the Ac treated one than His treated ( > ).
The combination of AC and His had the most protective effect against diabetes complications and advantageous effect on metabolism, β-cell activity, and insulin function due to the most reductive effect on the NF-κB pathway rather than More than any of the amino acids alone.
核因子-κB（NF-κB）信号传导参与糖尿病并发症。因此，降低NF-κB信号传导可能是预防或改善这些并发症的一个目标。于是，我们研究了乙酰半胱氨酸（AC）、组氨酸（His）及其组合对2型糖尿病大鼠NF-κB表达及其不同激活剂的影响。
对50只大鼠进行研究，将它们平均分为五组，即对照组、糖尿病组、用（AC，0.06%）治疗的糖尿病组、用（His，0.1%）治疗的糖尿病组和（AC & His）组。治疗组每天通过饮用水接受治疗，持续两个月。测量代谢指标（血糖、胰岛素抵抗指数、血脂谱和心血管指标）和肾功能不全参数（肌酐和尿蛋白排泄）。此外，还测定了不同的糖基化（早期、中期和晚期）、氧化应激（氧化型低密度脂蛋白、还原型谷胱甘肽）和炎症标志物（白细胞介素-1β、髓过氧化物酶和NF-κB表达）。
在（AC & His）治疗的糖尿病大鼠中，血糖、胰岛素抵抗指数、心血管指标、肾功能不全参数、不同的糖基化标志物、氧化应激和炎症以及NF-κB表达均最低。此外，AC治疗组的上述参数低于His治疗组（>）。
AC和His的组合对糖尿病并发症具有最强的保护作用，并且由于对NF-κB途径的还原作用最强，因此对代谢、β细胞活性和胰岛素功能具有有利影响，比单独使用任何一种氨基酸的效果都要好。
