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基于 mRNA 和小 RNA 测序分析感病茶树及其叶斑病原菌的分子互作。

Integrated mRNA and Small RNA Sequencing for Analyzing Leaf Spot Pathogen and Its Host, Tea (), During Infection.

机构信息

Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, Guizhou 550025, China.

Agricultural College, Guizhou University, Guiyang, Guizhou 550025, China.

出版信息

Mol Plant Microbe Interact. 2021 Jan;34(1):127-130. doi: 10.1094/MPMI-07-20-0207-A. Epub 2020 Nov 23.

DOI:10.1094/MPMI-07-20-0207-A
PMID:33021884
Abstract

Leaf spot on tea plants ( [L.] Kuntze), caused by the fungus (Q. Chen) Q. Chen, Crous & L. Cai (syn. ), negatively affects the productivity and quality of tea leaves in Guizhou Province, China. Although the genome sequence of has been published, no data on the transcriptome or microRNAs (miRNAs) of the pathogen or host during infection are available. Here, we report on the high-quality transcriptome and miRNA sequences of both and tea during infection, using the Illumina HiSeq 4000 or HiSeq 2500 platforms. Comprehensive expression profiling of the fungal pathogen and its host will provide a resource for future research into trait-specific genes of the pathogen and the host as well as on host-pathogen interactions and on disease resistance mechanisms.[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

摘要

茶树叶片斑点病([L.] Kuntze)由真菌(Q. Chen)Q. Chen、Crous & L. Cai(同义名)引起,严重影响了中国贵州省茶叶的产量和质量。尽管已公布了该真菌的基因组序列,但有关其在感染过程中的转录组或 microRNAs(miRNAs)的数据尚不可用。在这里,我们使用 Illumina HiSeq 4000 或 HiSeq 2500 平台,报道了感染过程中真菌病原体和宿主的高质量转录组和 miRNA 序列。真菌病原体及其宿主的综合表达谱分析将为未来研究病原体和宿主的特征基因、宿主-病原体相互作用以及抗病机制提供资源。

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引用本文的文献

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2
Genome-wide identification and expression profiling of chitinase genes in tea ( (L.) O. Kuntze) under biotic stress conditions.生物胁迫条件下茶树((L.) O. Kuntze)几丁质酶基因的全基因组鉴定及表达谱分析
Physiol Mol Biol Plants. 2021 Feb;27(2):369-385. doi: 10.1007/s12298-021-00947-x. Epub 2021 Feb 19.